Publication detail
In-situ Observation of Lyophilization Process in Environmental Scanning Electron Microscope
VETRÁKOVÁ, Ľ. NEDĚLA, V. RUNŠTUK, J. TIHLAŘÍKOVÁ, E. IMRICHOVÁ, K.
Original Title
In-situ Observation of Lyophilization Process in Environmental Scanning Electron Microscope
Type
abstract
Language
English
Original Abstract
Lyophilization (freeze-drying) is nowadays the most common formulation strategy to prepare pharmaceuticals as in the solid state chemical or physical degradation reactions are inhibited or sufficiently decelerated. Here we present dynamical in-situ monitoring of the drying step in lyophilisation of 5% (w/w) BSA solution inside environmental scanning electron microscope (ESEM) under pharmaceutically relevant conditions. We observed the changes in microstructure of the protein during drying at the temperature of −20 °C and the pressure of 700 Pa.
Keywords
lyophilization, BSA, ESEM, freezing
Authors
VETRÁKOVÁ, Ľ.; NEDĚLA, V.; RUNŠTUK, J.; TIHLAŘÍKOVÁ, E.; IMRICHOVÁ, K.
Released
10. 10. 2018
Location
Saarbrücken, Germany
Pages from
114
Pages to
115
Pages count
2
BibTex
@misc{BUT152657,
author="Ľubica {Vetráková} and Vilém {Neděla} and Jiří {Runštuk} and Eva {Tihlaříková} and Kamila {Závacká}",
title="In-situ Observation of Lyophilization Process in Environmental Scanning Electron Microscope",
year="2018",
pages="114--115",
address="Saarbrücken, Germany",
note="abstract"
}