Publication detail

Computational Design of Stable and Soluble Biocatalysts

MUSIL, M. KONEGGER, H. HON, J. BEDNÁŘ, D. DAMBORSKÝ, J.

Original Title

Computational Design of Stable and Soluble Biocatalysts

Type

journal article in Web of Science

Language

English

Original Abstract

Natural enzymes are delicate biomolecules possessing only marginal thermodynamic stability. Poorly stable, misfolded, and aggregated proteins lead to huge economic losses in the biotechnology and biopharmaceutical industries. Consequently, there is a need to design optimized protein sequences that maximize stability, solubility, and activity over a wide range of temperatures and pH values, in buffers of different composition, and in the presence of organic co-solvents. This has created great interest in using computational methods to enhance biocatalysts robustness and solubility. Suitable methods include (i) energy calculations, (ii) machine learning, (iii) phylogenetic analyses and (iv) combinations of these approaches. We have witnessed impressive progress in the design of stable enzymes over the last two decades, but predictions of protein solubility and expressibility are scarce. Stabilizing mutations can be predicted accurately using available force fields, the number of sequences available for phylogenetic analyses is growing, and complex computational workflows are being implemented in intuitive web tools, enhancing the quality of protein stability predictions. Conversely, solubility predictors are limited by the lack of robust and balanced experimental data, an inadequate understanding of fundamental principles of protein aggregation, and a dearth of structural information on folding intermediates. Here we summarize recent progress in the development of computational tools for predicting protein stability and solubility, critically assess their strengths and weaknesses, and identify apparent gaps in data and knowledge. We also present perspectives on the computational design of stable and soluble biocatalysts.

Keywords

Aggregation,Computational Design,Force Field,Expressibility,Machine Learning,Phylogenetic Analysis,Enzyme Stability,Enzyme Solubility

Authors

MUSIL, M.; KONEGGER, H.; HON, J.; BEDNÁŘ, D.; DAMBORSKÝ, J.

Released

1. 2. 2019

ISBN

2155-5435

Periodical

ACS Catalysis

Year of study

9

Number

2

State

United States of America

Pages from

1033

Pages to

1054

Pages count

22

URL

https://pubs.acs.org/doi/10.1021/acscatal.8b03613

BibTex

@article{BUT155120,
  author="Miloš {Musil} and Hannes {Konegger} and Jiří {Hon} and David {Bednář} and Jiří {Damborský}",
  title="Computational Design of Stable and Soluble Biocatalysts",
  journal="ACS Catalysis",
  year="2019",
  volume="9",
  number="2",
  pages="1033--1054",
  doi="10.1021/acscatal.8b03613",
  issn="2155-5435",
  url="https://pubs.acs.org/doi/10.1021/acscatal.8b03613"
}