Publication detail

Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level

PATÁKOVÁ, P. BRANSKÁ, B. SEDLÁŘ, K. VASYLKIVSKA, M. JUREČKOVÁ, K. KOLEK, J. CICKOVÁ, P. PROVAZNÍK, I.

Original Title

Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level

Type

journal article in Web of Science

Language

English

Original Abstract

Clostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, acidogenesis and solventogenesis, from which the latter one can be coupled with sporulation. Thorough transcriptomic profiling during a complete life cycle and both metabolic phases completed with flow cytometry, microscopy and a metabolites analysis helped to find out key genes involved in particular cellular events. The description of genes/operons that are closely involved in metabolism or the cell cycle is a necessary condition for metabolic engineering of the strain and will be valuable for all C. beijerinckii strains and other Clostridial species. The study focused on glucose transport and catabolism, hydrogen formation, metabolic stress response, binary fission, motility/chemotaxis and sporulation, which resulted in the composition of the unique image reflecting clostridial population changes. Surprisingly, the main change in expression of individual genes was coupled with the sporulation start and not with the transition from acidogenic to solventogenic metabolism. As expected, solvents formation started at pH decrease and the accumulation of butyric and acetic acids in the cultivation medium.

Keywords

Clostridium beijerinckii; ABE fermentation; transcriptomic profiling; life cycle; sporulation

Authors

PATÁKOVÁ, P.; BRANSKÁ, B.; SEDLÁŘ, K.; VASYLKIVSKA, M.; JUREČKOVÁ, K.; KOLEK, J.; CICKOVÁ, P.; PROVAZNÍK, I.

Released

4. 2. 2019

Publisher

Springer Nature

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

9

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

21

Pages count

21

URL

http://link.springer.com/article/10.1038/s41598-018-37679-0

Full text in the Digital Library

http://hdl.handle.net/11012/179544

BibTex

@article{BUT155993,
  author="Petra {Patáková} and Barbora {Branská} and Karel {Sedlář} and Maryna {Vasylkivska} and Kateřina {Šabatová} and Jan {Kolek} and Pavlína {Cicková} and Valentine {Provazník}",
  title="Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level",
  journal="Scientific Reports",
  year="2019",
  volume="9",
  number="1",
  pages="1--21",
  doi="10.1038/s41598-018-37679-0",
  issn="2045-2322",
  url="http://link.springer.com/article/10.1038/s41598-018-37679-0"
}