Publication detail

Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays

FOJTŮ, M. BALVAN, J. RAUDENSKÁ, M. VIČAR, T. BOUŠA, D. SOFER, Z. MASAŘÍK, M. PUMERA, M.

Original Title

Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays

Type

journal article in Web of Science

Language

English

Original Abstract

Black phosphorus (BP) belongs to a group of 2D nanomaterials and nowadays attracts constantly increasing attention. Parallel to the growing utilization of BP nanomaterial increase also the requirements for the thorough comprehension of its potential impact on human and animal health. The aim of this study was to compare and discuss five assays commonly used for the cytotoxicity assessments of nanomaterials with a special focus on BP nanoparticles. A comprehensive survey of factors and pitfalls is provided that should be accounted for when assessing their toxicity and pointed to their inconsistency. BP might introduce various levels of interference during toxicity assessments depending on its concentration applied. More importantly, the BP toxicity evaluation was found to be influenced by the nature of assay chosen. These are based on different principles and do not have to assess all the cellular events equally. A commercial assay based on the measurement of protease activity was identified to be the most suitable for the BP toxicity assessment. Further, the benefit of time-lapse quantitative phase imaging for nanomaterial toxicity evaluation was highlighted. Unlike the conventional assessments it provides real-time analysis of the processes accompanying BP administration and enables to understand them deeper and in the context.

Keywords

2D nanomaterials; black phosphorus; cytotoxicity; holographic microscopy; interference; nanoparticles; viability

Authors

FOJTŮ, M.; BALVAN, J.; RAUDENSKÁ, M.; VIČAR, T.; BOUŠA, D.; SOFER, Z.; MASAŘÍK, M.; PUMERA, M.

Released

2. 1. 2019

ISBN

0947-6539

Periodical

CHEMISTRY-A EUROPEAN JOURNAL

Year of study

25

Number

1

State

Federal Republic of Germany

Pages from

349

Pages to

360

Pages count

13

URL

https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201804434

BibTex

@article{BUT158443,
  author="Michaela {Fojtů} and Jan {Balvan} and Martina {Raudenská} and Tomáš {Vičar} and Daniel {Bouša 	} and Zdeněk {Sofer} and Michal {Masařík} and Martin {Pumera}",
  title="Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays",
  journal="CHEMISTRY-A EUROPEAN JOURNAL",
  year="2019",
  volume="25",
  number="1",
  pages="349--360",
  doi="10.1002/chem.201804434",
  issn="0947-6539",
  url="https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201804434"
}