Publication detail

The influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoforms

PORUBIAKOVÁ, O. BOHÁLOVÁ, N. INGA, A. VADOVIČOVÁ, N. COUFAL, J. FOJTA, M. BRÁZDA, V.

Original Title

The influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoforms

Type

journal article in Web of Science

Language

English

Original Abstract

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis and senescence. The human TP52 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure in the transactivation potential of full-lenght and N-terminal truncated p43alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53alpha isoforms.

Keywords

p53 protein protein-DNA interaction transactivatin potentail

Authors

PORUBIAKOVÁ, O.; BOHÁLOVÁ, N.; INGA, A.; VADOVIČOVÁ, N.; COUFAL, J.; FOJTA, M.; BRÁZDA, V.

Released

14. 1. 2020

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

21

Number

1

State

Swiss Confederation

Pages from

1

Pages to

15

Pages count

12

URL

https://www.mdpi.com/1422-0067/21/1/127

Full text in the Digital Library

http://hdl.handle.net/11012/189388

BibTex

@article{BUT159576,
  author="Otília {Porubiaková} and Natália {Bohálová} and Alberto {Inga} and Natália {Vadovičová} and Jan {Coufal} and Miroslav {Fojta} and Václav {Brázda}",
  title="The influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoforms",
  journal="International Journal of Molecular Sciences",
  year="2020",
  volume="21",
  number="1",
  pages="1--15",
  doi="10.3390/ijms21010127",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/21/1/127"
}