Publication detail

Alternate current high voltage pulses induce apoptotic death in myocardium by irreversible electroporation

Caluori, G., Tadesco, M., Pesl, M., Pavlova, I., Cervinka D., Raiteri, R., Rotrekl, V., Starek, Z.

Original Title

Alternate current high voltage pulses induce apoptotic death in myocardium by irreversible electroporation

Type

conference paper

Language

English

Original Abstract

Background: Irreversible electroporation (IRE) holds the promise to revolutionize catheter ablation of arrhythmia, overcoming the limits of thermal-based therapies. A careful combination of pulse parameters, safety conditions and knowledge of cell death mechanism must be pursued to maximize the benefits of the envisioned therapies. Aims: Identify a combination of parameters to induce complete local cell death. Prove the feasibility, efficacy and safety of AC-IRE ablation. Characterize the cellular and tissue damages induced by the AC-IRE application. Methods: Three study models are employed. In the first one, a cuvette-based system is used to calibrate the optimal pair of electric field-number of pulses to induce complete cell death. In the second one, a microelectrode array (MEA) system is used in combination with calcium imaging to identify electrical and ionic changes in cardiac cells affected by the identified pulse combination. In the third one, a swine model was used to study underwent AC-IRE ablation in left atrium (pulmonary vein ostia, chamber walls) and intraventricular septum.

Keywords

irreversible electroporation; catheter ablation, animal models; epicardial intervention

Authors

Caluori, G., Tadesco, M., Pesl, M., Pavlova, I., Cervinka D., Raiteri, R., Rotrekl, V., Starek, Z.

Released

2. 9. 2019

ISBN

978-2-913923-38-6

Book

3rd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food and Environmental Technologies

Edition number

1

Pages from

1

Pages to

1

Pages count

1

BibTex

@inproceedings{BUT159720,
  author="Dalibor {Červinka} and Zdeněk {Stárek} and Guido {Caluori}",
  title="Alternate current high voltage pulses induce apoptotic death in myocardium by irreversible electroporation",
  booktitle="3rd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food and Environmental Technologies",
  year="2019",
  number="3",
  pages="1--1",
  isbn="978-2-913923-38-6"
}