Publication detail

Optical topography of rough surfaces using vortex localization of fluorescent markers

SCHOVÁNEK, P. BOUCHAL, P. BOUCHAL, Z.

Original Title

Optical topography of rough surfaces using vortex localization of fluorescent markers

Type

journal article in Web of Science

Language

English

Original Abstract

Measuring rough surfaces is challenging because the proven topographic methods are impaired by the adverse effects of diffuse light. In our method, the measured surface is marked by fluorescent nanobeads allowing a complete suppression of diffuse light by bandpass filtering. Light emitted by each fluorescent bead is shaped to a double-helix point spread function used for three-dimensional bead localization on the surface. This non-interferometric measurement of rough surface topography is implemented in a vibration resistant setup. The comparison of our method with vertical scanning interferometry shows that a commercial profiler is surpassed when ground glass surfaces with steep slopes are measured. (C) 2020 Optical Society of America

Keywords

optical vortices, nondiffracting beams, optical topography, localization microscopy

Authors

SCHOVÁNEK, P.; BOUCHAL, P.; BOUCHAL, Z.

Released

15. 8. 2020

Publisher

OPTICAL SOC AMER

Location

WASHINGTON

ISBN

1539-4794

Periodical

Optics Letters

Year of study

45

Number

16

State

United States of America

Pages from

4468

Pages to

4471

Pages count

4

URL

https://www.osapublishing.org/ol/abstract.cfm?uri=ol-45-16-4468

BibTex

@article{BUT164672,
  author="Petr {Schovánek} and Petr {Bouchal} and Zdeněk {Bouchal}",
  title="Optical topography of rough surfaces using vortex localization of fluorescent markers",
  journal="Optics Letters",
  year="2020",
  volume="45",
  number="16",
  pages="4468--4471",
  doi="10.1364/OL.392072",
  issn="1539-4794",
  url="https://www.osapublishing.org/ol/abstract.cfm?uri=ol-45-16-4468"
}