Publication detail

Study of cholesterol’s effect on the properties of catanionic vesicular systems: comparison of light-scattering results with ultrasonic and fluorescence spectroscopy

HAVLÍKOVÁ, M. SZABOVÁ, J. JUGL, A. MRAVCOVÁ, L. CHANG, C. HUANG, P. PEKAŘ, M. MRAVEC, F.

Original Title

Study of cholesterol’s effect on the properties of catanionic vesicular systems: comparison of light-scattering results with ultrasonic and fluorescence spectroscopy

Type

journal article in Web of Science

Language

English

Original Abstract

This work is focused on the study of properties associated with the effect of cholesterol levels on the stability of vesicular systems based on the ion pair amphiphile hexadecyltrimethylammonium-dodecylsulphate (HTMA-DS) at laboratory temperature. The HTMA-DS catanionic system was doped with dioctadecyldimethylammonium chloride in a 9:1 molar ratio and cholesterol in the amount of 0, 3, 13, 23, 33, 43, 53, 63, and 73 mol.% was added. In this system, the size distributions were studied using the dynamic light-scattering technique and the zeta potential was determined. These standard techniques were supplemented by ultrasonic and fluorescence spectroscopy techniques. Due to low stability and high opalescence of samples, spectral techniques were used only for the samples with cholesterol content above 23 mol.%. The results from High-Resolution Ultrasonic Spectroscopy and from Fluorescence Spectroscopy are in agreement. They equally point to a change in the amount of hydration water in the membrane, the largest amount of which is present in the samples with 43 and 53 mol.% cholesterol. Using the light-scattering technique, the short-term stability of prepared vesicular systems was also observed over the first 36 days. Obtained results confirmed that the most stable systems are those containing 43 or 53 mol.% of cholesterol.

Keywords

Ion pair amphiphile; Catanionic vesicles; Cholesterol; High-resolution ultrasonic spectroscopy; hydration water; Fluorescence spectroscopy.

Authors

HAVLÍKOVÁ, M.; SZABOVÁ, J.; JUGL, A.; MRAVCOVÁ, L.; CHANG, C.; HUANG, P.; PEKAŘ, M.; MRAVEC, F.

Released

20. 12. 2020

Publisher

Elsevier B.V.

Location

Holandsko

ISBN

0927-7757

Periodical

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Year of study

607

Number

1

State

Kingdom of the Netherlands

Pages from

1

Pages to

7

Pages count

7

URL

https://www.sciencedirect.com/science/article/pii/S0927775720311195?via%3Dihub

Full text in the Digital Library

http://hdl.handle.net/11012/196450

BibTex

@article{BUT164858,
  author="Martina {Havlíková} and Jana {Szabová} and Adam {Jugl} and Ludmila {Mravcová} and Chien-Hsiang {Chang} and Po-Sung {Huang} and Miloslav {Pekař} and Filip {Mravec}",
  title="Study of cholesterol’s effect on the properties of catanionic vesicular systems: comparison of light-scattering results with ultrasonic and fluorescence spectroscopy",
  journal="Colloids and Surfaces A: Physicochemical and Engineering Aspects",
  year="2020",
  volume="607",
  number="1",
  pages="1--7",
  doi="10.1016/j.colsurfa.2020.125526",
  issn="0927-7757",
  url="https://www.sciencedirect.com/science/article/pii/S0927775720311195?via%3Dihub"
}