Publication detail

Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

ŠINDELÁŘOVÁ, A. POŘÍZKA, P. MODLITBOVÁ, P. VRLÍKOVÁ, L. KISS, K. KAŠKA, M. PROCHAZKA, D. VRÁBEL, J. BUCHTOVÁ, M. KAISER, J.

Original Title

Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues

Type

journal article in Web of Science

Language

English

Original Abstract

The improving performance of the laser-induced breakdown spectroscopy (LIBS) triggered its utilization in the challenging topic of soft tissue analysis. Alterations of elemental content within soft tissues are commonly assessed and provide further insights in biological research. However, the laser ablation of soft tissues is a complex issue and demands a priori optimization, which is not straightforward in respect to a typical LIBS experiment. Here, we focus on implementing an internal standard into the LIBS elemental analysis of soft tissue samples. We achieve this by extending routine methodology for optimization of soft tissues analysis with a standard spiking method. This step enables a robust optimization procedure of LIBS experimental settings. Considering the implementation of LIBS analysis to the histological routine, we avoid further alterations of the tissue structure. Therefore, we propose a unique methodology of sample preparation, analysis, and subsequent data treatment, which enables the comparison of signal response from heterogenous matrix for different LIBS parameters. Additionally, a brief step-by-step process of optimization to achieve the highest signal-to-noise ratio (SNR) is described. The quality of laser–tissue interaction is investigated on the basis of the zinc signal response, while selected experimental parameters (e.g., defocus, gate delay, laser energy, and ambient atmosphere) are systematically modified.

Keywords

laser-induced breakdown spectroscopy; elemental mapping; soft tissue ablation; zinc; murine kidneys

Authors

ŠINDELÁŘOVÁ, A.; POŘÍZKA, P.; MODLITBOVÁ, P.; VRLÍKOVÁ, L.; KISS, K.; KAŠKA, M.; PROCHAZKA, D.; VRÁBEL, J.; BUCHTOVÁ, M.; KAISER, J.

Released

29. 1. 2021

Publisher

Sensors

ISBN

1424-8220

Periodical

SENSORS

Year of study

21

Number

3

State

Swiss Confederation

Pages from

1

Pages to

13

Pages count

13

URL

https://www.mdpi.com/1424-8220/21/3/900/htm

Full text in the Digital Library

http://hdl.handle.net/11012/196339

BibTex

@article{BUT169460,
  author="Anna {Šindelářová} and Pavel {Pořízka} and Pavlína {Modlitbová} and Lucie {Vrlíková} and Kateřina {Kiss} and Milan {Kaška} and David {Prochazka} and Jakub {Vrábel} and Marcela {Buchtová} and Jozef {Kaiser}",
  title="Methodology for the Implementation of Internal Standard to Laser-Induced Breakdown Spectroscopy Analysis of Soft Tissues",
  journal="SENSORS",
  year="2021",
  volume="21",
  number="3",
  pages="1--13",
  doi="10.3390/s21030900",
  issn="1424-8220",
  url="https://www.mdpi.com/1424-8220/21/3/900/htm"
}