Publication detail

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

FENG, J. ZHU, H. LUKEŠ, J. KORABEČNÁ, M. FOHLEROVÁ, Z. MEI, T. CHANG, H. NEUŽIL, P.

Original Title

Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

Type

journal article in Web of Science

Language

English

Original Abstract

Microcalorimetry has been widely used in measuring cellular metabolic heat to study bioprocesses, such as metabolism. However, it is still limited by insufficient sensitivity and system complexity, especially for the direct measurements of individual cells. Here, we present a droplet-based simple differential microcalorimetric system for determining the real-time energy balance of the ciliate protist, Paramecium caudatum. We utilized the platform to dynamically monitor its defensive behavior and measured the temperature change. Then we used heat balance equation and calculated corresponding dissipated power and energy with an ultimate resolution of kl14 nW. The results showed that the defensive behavior by the ejection of the trichocysts & rsquo; content consumed energy of kl0.75 mJ per cell, and the dissipated power was kl303.8 & micro;W. This differential microcalorimetric platform can be used to study the metabolic heat of protist metabolism as well as other individual cells, helping us to understand this widespread, yet little-known, biological phenomenon from a new perspective. (c) 2021 Elsevier B.V. All rights reserved.

Keywords

Microcalorimetry; Energy balance monitoring; Defense mechanism; Metabolic heat; Unicellular organism

Authors

FENG, J.; ZHU, H.; LUKEŠ, J.; KORABEČNÁ, M.; FOHLEROVÁ, Z.; MEI, T.; CHANG, H.; NEUŽIL, P.

Released

22. 4. 2021

Publisher

ELSEVIER SCIENCE SA

Location

LAUSANNE

ISBN

0924-4247

Periodical

Sensors and Actuators

Year of study

323

Number

1

State

Swiss Confederation

Pages from

1

Pages to

9

Pages count

9

URL

http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=3&SID=E44ZFBnYg876vQecPbL&page=1&doc=1

BibTex

@article{BUT171297,
  author="Jianguo {Feng} and Hanliang {Zhu} and Julius {Lukeš} and Marie {Korabečná} and Zdenka {Fohlerová} and Ting {Mei} and Honglong {Chang} and Pavel {Neužil}",
  title="Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum",
  journal="Sensors and Actuators",
  year="2021",
  volume="323",
  number="1",
  pages="1--9",
  doi="10.1016/j.sna.2021.112643",
  issn="0924-4247",
  url="http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=3&SID=E44ZFBnYg876vQecPbL&page=1&doc=1"
}