Publication detail

Production of Enriched Sporidiobolus sp. Yeast Biomass Cultivated on Mixed Coffee Hydrolyzate and Fat/Oil Waste Materials

SZOTKOWSKI, M. HOLUB, J. ŠIMANSKÝ, S. HUBAČOVÁ, K. HLADKÁ, D. NĚMCOVÁ, A. MÁROVÁ, I.

Original Title

Production of Enriched Sporidiobolus sp. Yeast Biomass Cultivated on Mixed Coffee Hydrolyzate and Fat/Oil Waste Materials

Type

journal article in Web of Science

Language

English

Original Abstract

One of the most addressed topics today is the transfer from a linear model of economics to a model of circular economics. It is a discipline that seeks to eliminate waste produced by various industries. The food industry generates huge amounts of waste worldwide, particularly the coffee industry, and related industries produce millions of tons of waste a year. These wastes have potential utility in biotechnology, and in the production of energy, fuels, fertilizers and nutrients, using green techniques such as anaerobic digestion, co-digestion, composting, enzymatic action, and ultrasonic and hydrothermal carbonization. This work is focused on the biotechnological use of processed spent coffee grounds (SCG) and waste fat/oil materials by some Sporidiobolus sp. carotenogenic yeasts in the model of circular economics. The results show that selected yeast strains are able to grow on SCG hydrolysate and are resistant to antimicrobial compounds present in media. The most productive strain Sporidiobolus pararoseus CCY19-9-6 was chosen for bioreactor cultivation in media with a mixture of coffee lignocellulose fraction and some fat wastes. Sporidiobolus pararoseus CCY19-9-6 was able to produce more than 22 g/L of biomass in mixture of SCG hydrolysate and both coffee oil and frying oil. The combined waste substrates induced the production of lipidic metabolites, whereby the production of carotenoids exceeded 5 mg/g of dry biomass. On media with coffee oil, this strain produced high amounts of ubiquinone (8.265 +/- 1.648 mg/g) and ergosterol (13.485 +/- 1.275 mg/g). Overall, the results prove that a combination of waste substrates is a promising option for the production of carotenoid- and lipid-enriched yeast biomass.

Keywords

carotenogenic yeasts; lipids; carotenoids; spent coffee grounds hydrolysate; waste animal fat; waste frying oil; coffee oil

Authors

SZOTKOWSKI, M.; HOLUB, J.; ŠIMANSKÝ, S.; HUBAČOVÁ, K.; HLADKÁ, D.; NĚMCOVÁ, A.; MÁROVÁ, I.

Released

31. 8. 2021

Publisher

MDPI

Location

BASEL

ISBN

2076-2607

Periodical

Microorganisms

Year of study

9

Number

9

State

Swiss Confederation

Pages from

1

Pages to

21

Pages count

21

URL

https://www.mdpi.com/2076-2607/9/9/1848

Full text in the Digital Library

http://hdl.handle.net/11012/202242

BibTex

@article{BUT172748,
  author="Martin {Szotkowski} and Jiří {Holub} and Samuel {Šimanský} and Klára {Hubačová} and Dagmar {Hladká} and Andrea {Němcová} and Ivana {Márová}",
  title="Production of Enriched Sporidiobolus sp. Yeast Biomass Cultivated on Mixed Coffee Hydrolyzate and Fat/Oil Waste Materials",
  journal="Microorganisms",
  year="2021",
  volume="9",
  number="9",
  pages="1--21",
  doi="10.3390/microorganisms9091848",
  issn="2076-2607",
  url="https://www.mdpi.com/2076-2607/9/9/1848"
}