Publication detail

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

HANKEOVÁ, S. ŠALPLACHTA, J. VAN HUL, N. KAVKOVÁ, M. IQBAL, A. ZIKMUND, T. KAISER, J. ANDERSSON, E.

Original Title

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Type

journal article in Web of Science

Language

English

Original Abstract

The liver is the biggest internal organ in humans and mice, and high auto-fluorescence presents a significant challenge for assessing the three-dimensional (3D) architecture of the organ at the whole-organ level. Liver architecture is characterized by multiple branching lumenized structures, which can be filled with resin, including vascular and biliary trees, establishing a highly stereotyped pattern in the otherwise hepatocyte-rich parenchyma. This protocol describes the pipeline for performing double resin casting micro-computed tomography, or "DUCT". DUCT entails injecting the portal vein and common bile duct with two different radiopaque synthetic resins, followed by tissue fixation. Quality control by clearing one lobe, or the entire liver, with an optical clearing agent, allows for pre-screening of suitably injected samples. In the second part of the DUCT pipeline, a lobe or the whole liver can be used for micro-computed tomography (microCT) scanning, (semi-)automated segmentation, and 3D rendering of the portal venous and biliary networks. MicroCT results in 3D coordinate data for the two resins allowing for qualitative as well as quantitative analysis of the two systems and their spatial relationship. DUCT can be applied to postnatal and adult mouse liver and can be further extended to other tubular networks, for example, vascular networks and airways in the lungs.

Keywords

micro CT, liver analysis, DUCT

Authors

HANKEOVÁ, S.; ŠALPLACHTA, J.; VAN HUL, N.; KAVKOVÁ, M.; IQBAL, A.; ZIKMUND, T.; KAISER, J.; ANDERSSON, E.

Released

28. 9. 2021

Publisher

MYJoVE Corporation

Location

United States

ISBN

1940-087X

Periodical

Journal of visualized experiments : JoVE

Year of study

175

Number

e62941

State

United States of America

Pages from

1

Pages to

19

Pages count

19

URL

https://www.jove.com/t/62941/duct-double-resin-casting-followed-micro-computed-tomography-for-3d

Full text in the Digital Library

http://hdl.handle.net/11012/203065

BibTex

@article{BUT172883,
  author="Simona {Hankeová} and Jakub {Šalplachta} and Noemi {Van Hul} and Michaela {Kavková} and Afsan {Iqbal} and Tomáš {Zikmund} and Jozef {Kaiser} and Emma Rachel {Andersson}",
  title="DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis",
  journal="Journal of visualized experiments : JoVE",
  year="2021",
  volume="175",
  number="e62941",
  pages="1--19",
  doi="10.3791/62941",
  issn="1940-087X",
  url="https://www.jove.com/t/62941/duct-double-resin-casting-followed-micro-computed-tomography-for-3d"
}