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NOVÁČKOVÁ, I. KOUŘILOVÁ, X. MRÁZOVÁ, K. OBRUČA, S.
Original Title
Environmentally-friendly Isolation of Polyhydroxyalkanoates from Extremophilic Bacteria
Type
abstract
Language
English
Original Abstract
Biotechnological production of PHA employing extremophiles within concept of Next-Generation Industrial Biotechnology (NGIB) bring many benefits to whole process considering requirement for sterility, cultivation, and also specific possibilities for PHA isolation process. Within our work, we focused on two extremophilic producers, halophilic bacterium Halomonas halophila (CCM 3662) and thermophilic bacterial strain Schlegelella thermodepolymerans (DSM 15344). Unique features of selected extremophiles are used for development of environmentally friendly and also economically feasible isolation approach combining hypotonic shock, elevated temperature and detergent sodium dodecyl sulphate (SDS), which can lead to PHB with purity higher than 90 wt.%. Moreover, we will focuse on recyclation of residual SDS, which represents pollutant of wastewater after isolation.
Keywords
PHA, SDS, Halomonas halophila, Schlegelella thermodepolymerans, NGIB
Authors
NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; MRÁZOVÁ, K.; OBRUČA, S.
Released
1. 12. 2021
ISBN
2564-615X
Periodical
The EuroBiotech Journal
Year of study
5
Number
2
State
Republic of Italy
Pages from
99
Pages to
Pages count
1
URL
https://sciendo.com/issue/EBTJ/5/s2
BibTex
@misc{BUT173031, author="Ivana {Nováčková} and Xenie {Kouřilová} and Kateřina {Mrázová} and Stanislav {Obruča}", title="Environmentally-friendly Isolation of Polyhydroxyalkanoates from Extremophilic Bacteria", year="2021", journal="The EuroBiotech Journal", volume="5", number="2", pages="99--99", issn="2564-615X", url="https://sciendo.com/issue/EBTJ/5/s2", note="abstract" }