Publication detail

Amine modification of calcium phosphate by low-pressure plasma for bone regeneration

KODAMA, J. HARUMNINGTYAS, A. ITO, T. MICHLÍČEK, M. SUGIMOTO, S. KITA, H. CHIJIMATSU, R. UKON, Y. KUSHIOKA, J. OKADA, R. KAMATANI, T. HASHIMOTO, K. TATEIWA, D. TSUKAZAKI, H. NAKAGAWA, S. TAKENAKA, S. MAKINO, T. SAKAI, Y. NEČAS, D. ZAJÍČKOVÁ, L. HAMAGUCHI, S. KAITO, T.

Original Title

Amine modification of calcium phosphate by low-pressure plasma for bone regeneration

Type

journal article in Web of Science

Language

English

Original Abstract

Regeneration of large bone defects caused by trauma or tumor resection remains one of the biggest challenges in orthopedic surgery. Because of the limited availability of autograft material, the use of artificial bone is prevalent; however, the primary role of currently available artificial bone is restricted to acting as a bone graft extender owing to the lack of osteogenic ability. To explore whether surface modification might enhance artificial bone functionality, in this study we applied low-pressure plasma technology as next-generation surface treatment and processing strategy to chemically (amine) modify the surface of beta-tricalcium phosphate (beta-TCP) artificial bone using a CH4/N-2/He gas mixture. Plasma-treated beta-TCP exhibited significantly enhanced hydrophilicity, facilitating the deep infiltration of cells into interconnected porous beta-TCP. Additionally, cell adhesion and osteogenic differentiation on the plasma-treated artificial bone surfaces were also enhanced. Furthermore, in a rat calvarial defect model, the plasma treatment afforded high bone regeneration capacity. Together, these results suggest that amine modification of artificial bone by plasma technology can provide a high osteogenic ability and represents a promising strategy for resolving current clinical limitations regarding the use of artificial bone.

Keywords

mesenchymal stem-cells; osteoblast differentiation; integrin binding; in-vitro; adhesion; fibronectin; polymerization; specificity; topography; activation

Authors

KODAMA, J.; HARUMNINGTYAS, A.; ITO, T.; MICHLÍČEK, M.; SUGIMOTO, S.; KITA, H.; CHIJIMATSU, R.; UKON, Y.; KUSHIOKA, J.; OKADA, R.; KAMATANI, T.; HASHIMOTO, K.; TATEIWA, D.; TSUKAZAKI, H.; NAKAGAWA, S.; TAKENAKA, S.; MAKINO, T.; SAKAI, Y.; NEČAS, D.; ZAJÍČKOVÁ, L.; HAMAGUCHI, S.; KAITO, T.

Released

1. 9. 2021

Publisher

Nature Portfolio

Location

BERLIN

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

11

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

15

Pages count

15

URL

https://www.nature.com/articles/s41598-021-97460-8

Full text in the Digital Library

http://hdl.handle.net/11012/203224

BibTex

@article{BUT174111,
  author="Joe {Kodama} and Anjar Anggraini {Harumningtyas} and Tomoko {Ito} and Miroslav {Michlíček} and Satoshi {Sugimoto} and Hidekazu {Kita} and Ryota {Chijimatsu} and Yuichiro {Ukon} and Junichi {Kushioka} and Rintaro {Okada} and Takashi {Kamatani} and Kunihiko {Hashimoto} and Daisuke {Tateiwa} and Hiroyuki {Tsukazaki} and Shinichi {Nakagawa} and Shota {Takenaka} and Takahiro {Makino} and Yusuke {Sakai} and David {Nečas} and Lenka {Zajíčková} and Satoshi {Hamaguchi} and Takashi {Kaito}",
  title="Amine modification of calcium phosphate by low-pressure plasma for bone regeneration",
  journal="Scientific Reports",
  year="2021",
  volume="11",
  number="1",
  pages="1--15",
  doi="10.1038/s41598-021-97460-8",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-021-97460-8"
}