Publication detail

Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques

HOLÍNKOVÁ, P. MRAVEC, F. VENEROVÁ, T. CHANG, C. PEKAŘ, M.

Original Title

Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques

Type

journal article in Web of Science

Language

English

Original Abstract

Interactions of hyaluronan with micelles formed by cationic surfactants were studied by the time-resolved measurement of fluorescence resonance energy transfer (FRET) using perylene and fluorescein as probes. Two surfactants were studied - Cetyltrimethylammonium bromide (CTAB) and Septonex. In pure micellar solutions, the same values of FRET efficiency were found for both surfactants, but values for the binding of the probe pair were lower for Septonex micelles than in the case of CTAB. This was attributed to steric effects of the carbethoxy group in the Septonex polar head. Upon the addition of hyaluronan, decreased FRET efficiency and increased binding were detected in comparison with pure surfactants. To resolve the structure of the formed aggregates, steady state and time-resolved fluorescence anisotropy was employed as an additional technique. All results indicated that cationic micelles bind to hyaluronan forming a pearl necklace structure with micelles of smaller size compared to pure surfactant. Besides theoretical interest, the studied polyelectrolyte-surfactant system may be interesting for the formulation of drug delivery systems.

Keywords

Fluorescence anisotropy; Fo ?rster resonance energy transfer; Hyaluronan; Surfactants

Authors

HOLÍNKOVÁ, P.; MRAVEC, F.; VENEROVÁ, T.; CHANG, C.; PEKAŘ, M.

Released

30. 6. 2022

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

211

Number

1

State

Kingdom of the Netherlands

Pages from

107

Pages to

115

Pages count

9

URL

https://www.sciencedirect.com/science/article/abs/pii/S0141813022010376?via%3Dihub

BibTex

@article{BUT178267,
  author="Petra {Holínková} and Filip {Mravec} and Tereza {Venerová} and Chien-Hsiang {Chang} and Miloslav {Pekař}",
  title="Hyaluronan interactions with cationic surfactants-Insights from fluorescence resonance energy transfer and anisotropy techniques",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2022",
  volume="211",
  number="1",
  pages="107--115",
  doi="10.1016/j.ijbiomac.2022.05.067",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/abs/pii/S0141813022010376?via%3Dihub"
}