Publication detail

3D Printed calcium phosphate cement scaffolds loaded with liposomal antibacterial enzymes for the prevention of osteomyelitis

KADLECOVÁ, Z. HLINÁKOVÁ, K. VOJTOVÁ, L.

Original Title

3D Printed calcium phosphate cement scaffolds loaded with liposomal antibacterial enzymes for the prevention of osteomyelitis

Type

abstract

Language

English

Original Abstract

To treat large-scale bone defects, three-dimensional (3D) printing has been in focus in recent years due to the possibility of fabricating patient-specific bioactive and porous scaffolds1 . When the 3D printed implant begins to resorb, the osteointegration process is often accompanied by several microorganisms that cause a bone infection known as osteomyelitis2 . By combining the ceramic phosphate powder, thermosensitive copolymer, and enzymes, we obtain the bio-ink suitable for 3D printing of bone implants with controllable degradation rate, mechanical properties, pore size, and antibacterial activity. Therefore, as the calcium phosphate cement (CPC) implant resorbs, it suppresses the growth of undesirable microorganisms. Our previous study discovered that antibacterial enzymes incorporated into the bio-ink do not release from the implants in more significant amounts than expected. This probably results from the CPC structure as the enzymes are “locked” within the CPC structure. In this project, the antibacterial enzyme Lysozyme is encapsulated into liposomes with diameters around 100 nm, providing additional chemical and physical protection to the enzyme during the implant preparation and curing. Liposomes prepared by an optimized “heating method” exhibit a low polydispersity index and diameters around 97.8±4.8 nm and zeta potential of -15.2±3.2 mV (n=3). The encapsulation efficiency of Lysozyme in these formulas is around 64 %. It was determined that Lysozyme is released from such liposomes in a first-order release mechanism. The release of the liposome-Lysozyme system from the CPC scaffolds is currently being evaluated.

Keywords

Calcium phosphate cement, liposomes, osteomyelitis, Lysozyme

Authors

KADLECOVÁ, Z.; HLINÁKOVÁ, K.; VOJTOVÁ, L.

Released

29. 8. 2022

Publisher

Materials Research Society of Serbia

Location

Belgrade

ISBN

978-86-919111-7-1

Book

Program and Book of Abstracts TWENTY-THIRD ANNUAL CONFERENCE YUCOMAT 2022 & TWELFTH WORLD ROUND TABLE CONFERENCE ON SINTERING XII WRTCS

Edition

1

Pages from

98

Pages to

98

Pages count

1

URL

https://www.mrs-serbia.org.rs/images/2022/YUCOMAT_2022-XII_WRTCS.pdf

BibTex

@misc{BUT179243,
  author="Zuzana {Kadlecová} and Kristýna {Hlináková} and Lucy {Vojtová}",
  title="3D Printed calcium phosphate cement scaffolds loaded with liposomal antibacterial
enzymes for the prevention of osteomyelitis",
  booktitle="Program and Book of Abstracts
TWENTY-THIRD ANNUAL CONFERENCE YUCOMAT 2022 &
TWELFTH WORLD ROUND TABLE CONFERENCE ON SINTERING XII WRTCS",
  year="2022",
  series="1",
  pages="98--98",
  publisher="Materials Research Society of Serbia",
  address="Belgrade",
  isbn="978-86-919111-7-1",
  url="https://www.mrs-serbia.org.rs/images/2022/YUCOMAT_2022-XII_WRTCS.pdf",
  note="abstract"
}