Dual-focus fluorescence correlation spectroscopy in the study of hydrogel properties

abstract

English

This contribution describes dependency of nanoparticles diffusion in the agarose hydrogel on the hydrogel concentration. The description of Brownian motion, in this case diffusion coefficient, can be measured by many techniques. Main disadvantage is the need to have a large amount of sample. For fluorescence correlation spectroscopy (FCS) techniques, it is necessary to have only few microliters of nanomolar concentration of the sample, also the confocal volume allows measurement of pretty small amount of the sample. Unlike the single-FCS method, calibration is not required in dualfocus each time the temperature changes. Based on the optimization of the method and previous measurements, a suitable procedure for measuring the agarose samples was chosen. All data obtained from dual-focus FCS were compared with data measured using single-focus FCS. It was found, nanoparticles in water had very similar diffusion coefficient value. Significant differences were have been seen even at the lowest hydrogel concentration. Some of the autocorrelation curves had different shape compared with autocorrelation curves obtain form single-focus FCS.

dual-focus FCS; hydrogel; fluorescence correlation spectrocopy; nanoparticles

MARKOVÁ, K.; PEKAŘ, M.

26. 11. 2021

Vysoké učení technické v Brně, Fakulta chemická

Brno

978-80-214-6002-7

Studentská odborná konference Chemie je život 2021

první

88

88

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