Publication detail

Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry

JUGL, A. PEKAŘ, M.

Original Title

Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry

Type

journal article in Web of Science

Language

English

Original Abstract

Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed.

Keywords

Hyaluronan; Cecropin B; Interaction; High-resolution ultrasonic spectroscopy; Isothermal titration calorimetry

Authors

JUGL, A.; PEKAŘ, M.

Released

1. 2. 2023

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

227

Number

1

State

Kingdom of the Netherlands

Pages from

786

Pages to

794

Pages count

9

URL

https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub

BibTex

@article{BUT180491,
  author="Adam {Jugl} and Miloslav {Pekař}",
  title="Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2023",
  volume="227",
  number="1",
  pages="786--794",
  doi="10.1016/j.ijbiomac.2022.12.144",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S014181302203046X?via%3Dihub"
}