Publication detail

A mid-infrared lab-on-a-chip for dynamic reaction monitoring

HINKOV, B. PILAT, F. LUX, L. SOUZA, P. DAVID, M. SCHWAIGHOFER, A. RISTANIC, D. SCHWARZ, B. DETZ, H. ANDREWS, A. LENDL, B. STRASSER, G.

Original Title

A mid-infrared lab-on-a-chip for dynamic reaction monitoring

Type

journal article in Web of Science

Language

English

Original Abstract

Mid-infrared spectroscopy is a sensitive and selective technique for probing molecules in the gas or liquid phase. Investigating chemical reactions in bio-medical applications such as drug production is recently gaining particular interest. However, monitoring dynamic processes in liquids is commonly limited to bulky systems and thus requires time-consuming offline analytics. In this work, we show a next-generation, fully-integrated and robust chip-scale sensor for online measurements of molecule dynamics in a liquid solution. Our fingertip-sized device utilizes quantum cascade technology, combining the emitter, sensing section and detector on a single chip. This enables real-time measurements probing only microliter amounts of analyte in an in situ configuration. We demonstrate time-resolved device operation by analyzing temperature-induced conformational changes of the model protein bovine serum albumin in heavy water. Quantitative measurements reveal excellent performance characteristics in terms of sensor linearity, wide coverage of concentrations, extending from 0.075 mg ml(-1) to 92 mg ml(-1) and a 55-times higher absorbance than state-of-the-art bulky and offline reference systems. Rapid investigation of chemical reactions is a challenge in bio-medical analysis. Here, the authors demonstrate sensitive in-situ real-time reaction-monitoring of conformational changes in protein solution, based on a fingertip-sized mid-IR lab-on-a-chip.

Keywords

BOVINE SERUM-ALBUMIN; QUANTUM-CASCADE-LASER; INFRARED FT-IR; DISTRIBUTED-FEEDBACK; SECONDARY STRUCTURE; SPECTROSCOPY; SENSORS; CONFORMATION; MODULATION; PROTEINS

Authors

HINKOV, B.; PILAT, F.; LUX, L.; SOUZA, P.; DAVID, M.; SCHWAIGHOFER, A.; RISTANIC, D.; SCHWARZ, B.; DETZ, H.; ANDREWS, A.; LENDL, B.; STRASSER, G.

Released

13. 8. 2022

Publisher

NATURE PORTFOLIO

Location

BERLIN

ISBN

2041-1723

Periodical

NATURE COMMUNICATIONS

Year of study

13

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages count

10

URL

https://www.nature.com/articles/s41467-022-32417-7

BibTex

@article{BUT181647,
  author="Borislav {Hinkov} and Florian {Pilat} and Laurin {Lux} and Patricia L. {Souza} and Mauro {David} and Andreas {Schwaighofer} and Daniela {Ristanic} and Benedikt {Schwarz} and Hermann {Detz} and Aaron M. {Andrews} and Bernhard {Lendl} and Gottfried {Strasser}",
  title="A mid-infrared lab-on-a-chip for dynamic reaction monitoring",
  journal="NATURE COMMUNICATIONS",
  year="2022",
  volume="13",
  number="1",
  pages="10",
  doi="10.1038/s41467-022-32417-7",
  issn="2041-1723",
  url="https://www.nature.com/articles/s41467-022-32417-7"
}