Publication detail

Poly(3-hydroxybutyrate) (PHB) and Polycaprolactone (PCL) Based Blends for Tissue Engineering and Bone Medical Applications Processed by FDM 3D Printing

KROBOT, Š. MELČOVÁ, V. MENČÍK, P. KONTÁROVÁ, S. RAMPICHOVÁ, M. HEDVIČÁKOVÁ, V. MOJŽIŠOVÁ, E. BACO, A. PŘIKRYL, R.

Original Title

Poly(3-hydroxybutyrate) (PHB) and Polycaprolactone (PCL) Based Blends for Tissue Engineering and Bone Medical Applications Processed by FDM 3D Printing

Type

journal article in Web of Science

Language

English

Original Abstract

In the presented work, poly(3-hydroxybutyrate)-PHB-based composite blends for bone medical applications and tissue engineering are prepared and characterized. PHB used for the work was in two cases commercial and, in one case, was extracted by the chloroform-free route. PHB was then blended with poly(lactic acid) (PLA) or polycaprolactone (PCL) and plasticized by oligomeric adipate ester (Syncroflex, SN). Tricalcium phosphate (TCP) particles were used as a bioactive filler. Prepared polymer blends were processed into the form of 3D printing filaments. The samples for all the tests performed were prepared by FDM 3D printing or compression molding. Differential scanning calorimetry was conducted to evaluate the thermal properties, followed by optimization of printing temperature by temperature tower test and determination of warping coefficient. Tensile test, three-point flexural test, and compression test were performed to study the mechanical properties of materials. Optical contact angle measurement was conducted to determine the surface properties of these blends and their influence on cell adhesion. Cytotoxicity measurement of prepared blends was conducted to find out whether the prepared materials were non-cytotoxic. The best temperatures for 3D printing were 195/190, 195/175, and 195/165 degrees C for PHB-soap/PLA-SN, PHB/PCL-SN, and PHB/PCL-SN-TCP, respectively. Their mechanical properties (strengths similar to 40 MPa, moduli similar to 2.5 GPa) were comparable with human trabecular bone. The calculated surface energies of all blends were similar to 40 mN/m. Unfortunately, only two out of three materials were proven to be non-cytotoxic (both PHB/PCL blends).

Keywords

3D printing; FDM; poly(3-hydroxybutyrate); bone tissue engineering; scaffold; biocompatibility; polycaprolactone; polylactic acid

Authors

KROBOT, Š.; MELČOVÁ, V.; MENČÍK, P.; KONTÁROVÁ, S.; RAMPICHOVÁ, M.; HEDVIČÁKOVÁ, V.; MOJŽIŠOVÁ, E.; BACO, A.; PŘIKRYL, R.

Released

22. 5. 2023

Publisher

MDPI

Location

BASEL

ISBN

2073-4360

Periodical

Polymers

Year of study

15

Number

10

State

Swiss Confederation

Pages from

1

Pages to

17

Pages count

17

URL

https://www.mdpi.com/2073-4360/15/10/2404

Full text in the Digital Library

http://hdl.handle.net/11012/209755

BibTex

@article{BUT183556,
  author="Štěpán {Krobot} and Veronika {Melčová} and Přemysl {Menčík} and Soňa {Kontárová} and Michaela {Rampichová} and Věra {Hedvičáková} and Ema {Mojžišová} and Andrej {Baco} and Radek {Přikryl}",
  title="Poly(3-hydroxybutyrate) (PHB) and Polycaprolactone (PCL) Based Blends for Tissue Engineering and Bone Medical Applications Processed by FDM 3D Printing",
  journal="Polymers",
  year="2023",
  volume="15",
  number="10",
  pages="1--17",
  doi="10.3390/polym15102404",
  issn="2073-4360",
  url="https://www.mdpi.com/2073-4360/15/10/2404"
}