Publication detail

Transcriptomic hallmarks of in vitro TiO2 nanotubes toxicity in Chlamydomonas reinhardtii

DOBEŠOVÁ, M. KOLÁČKOVÁ, M. PĚNČÍK, O. CÁPAL, P. CHALOUPSKÝ, P. ŠVEC, P. RIDOŠKOVÁ, A. MOTOLA, M. ČIČMANCOVÁ, V. SOPHA, H. MACÁK, J. RICHTERA, L. ADAM, V. HÚSKA, D.

Original Title

Transcriptomic hallmarks of in vitro TiO2 nanotubes toxicity in Chlamydomonas reinhardtii

Type

journal article in Web of Science

Language

English

Original Abstract

Recently, more accessible transcriptomic approaches have provided a new and deeper understanding of envi- ronmental toxicity. The present study focuses on the transcriptomic profiles of green microalgae Chlamydomonas reinhardtii exposed to new industrially promising material, TiO2 nanotubes (NTs), as an example of a widely used one-dimensional nanomaterial. The first algal in vitro assay included 2.5 and 7.5 mg/L TiO2 NTs, resulting in a dose-dependent negative effect on biological endpoints. At a working concentration of 7.5 mg/L, RNA - sequencing showed a mainly negative effect on the cells. In summary, the results indicated metabolic disrup- tion, such as ATP loss, damage to mitochondria and chloroplasts, loss of solutes due to permeated membranes, and cell wall damage. Moreover, apoptosis-induced transcripts were detected. Interestingly, reactivation of transposons was observed. In signalling and transcription pathways, including chromatin remodelling and locking, the annotated genes were downregulated.

Keywords

TiO2; Nanotubes; Toxicity; Chlamydomonas; RNA-seq

Authors

DOBEŠOVÁ, M.; KOLÁČKOVÁ, M.; PĚNČÍK, O.; CÁPAL, P.; CHALOUPSKÝ, P.; ŠVEC, P.; RIDOŠKOVÁ, A.; MOTOLA, M.; ČIČMANCOVÁ, V.; SOPHA, H.; MACÁK, J.; RICHTERA, L.; ADAM, V.; HÚSKA, D.

Released

1. 3. 2023

Publisher

ELSEVIER

Location

AMSTERDAM

ISBN

1879-1514

Periodical

Aquatic Toxicology

Year of study

256

Number

106419

State

Kingdom of the Netherlands

Pages count

14

URL

https://www.sciencedirect.com/science/article/pii/S0166445X23000267?via%3Dihub

BibTex

@article{BUT184133,
  author="Markéta {Dobešová} and Martina {Koláčková} and Ondřej {Pěnčík} and Petr {Cápal} and Pavel {Chaloupský} and Pavel {Švec} and Andrea {Ridošková} and Martin {Motola} and Veronika {Čičmancová} and Hanna Ingrid {Sopha} and Jan {Macák} and Lukáš {Richtera} and Vojtěch {Adam} and Dalibor {Húska}",
  title="Transcriptomic hallmarks of in vitro TiO2 nanotubes toxicity in Chlamydomonas reinhardtii",
  journal="Aquatic Toxicology",
  year="2023",
  volume="256",
  number="106419",
  pages="14",
  doi="10.1016/j.aquatox.2023.106419",
  issn="1879-1514",
  url="https://www.sciencedirect.com/science/article/pii/S0166445X23000267?via%3Dihub"
}