Publication detail

Antimicrobial Activity of Gelatin Nanofibers Enriched by Essential Oils against Cutibacterium acnes and Staphylococcus epidermidis

UHLÍŘOVÁ, R. LANGOVÁ, D. BENDOVÁ, A. GROSS, M. SKOUMALOVÁ, P. MÁROVÁ, I.

Original Title

Antimicrobial Activity of Gelatin Nanofibers Enriched by Essential Oils against Cutibacterium acnes and Staphylococcus epidermidis

Type

journal article in Web of Science

Language

English

Original Abstract

Acne vulgaris is a prevalent skin condition that is caused by an imbalance in skin microbiomes mainly by the overgrowth of strains such as Cutibacterium acnes and Staphylococcus epidermidis which affect both teenagers and adults. Drug resistance, dosing, mood alteration, and other issues hinder traditional therapy. This study aimed to create a novel dissolvable nanofiber patch containing essential oils (EOs) from Lavandula angustifolia and Mentha piperita for acne vulgaris treatment. The EOs were characterized based on antioxidant activity and chemical composition using HPLC and GC/MS analysis. The antimicrobial activity against C. acnes and S. epidermidis was observed by the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The MICs were in the range of 5.7-9.4 mu L/mL, and MBCs 9.4-25.0 mu L/mL. The EOs were integrated into gelatin nanofibers by electrospinning and SEM images of the fibers were taken. Only the addition of 20% of pure essential oil led to minor diameter and morphology alteration. The agar diffusion tests were performed. Pure and diluted Eos in almond oil exhibited a strong antibacterial effect on C. acnes and S. epidermidis. After incorporation into nanofibers, we were able to focus the antimicrobial effect only on the spot of application with no effect on the surrounding microorganisms. Lastly, for cytotoxicity evaluation, and MTT assay was performed with promising results that samples in the tested range had a low impact on HaCaT cell line viability. In conclusion, our gelatin nanofibers containing EOs are suitable for further investigation as prospective antimicrobial patches for acne vulgaris local treatment.

Keywords

nanofibers; antimicrobial activity; acne vulgaris; essential oils; Lavandula angustifolia; Mentha piperita; local treatment

Authors

UHLÍŘOVÁ, R.; LANGOVÁ, D.; BENDOVÁ, A.; GROSS, M.; SKOUMALOVÁ, P.; MÁROVÁ, I.

Released

22. 3. 2023

Publisher

MDPI

Location

BASEL

ISBN

2079-4991

Periodical

Nanomaterials

Year of study

13

Number

5

State

Swiss Confederation

Pages from

1

Pages to

18

Pages count

18

URL

https://www.mdpi.com/2079-4991/13/5/844

Full text in the Digital Library

http://hdl.handle.net/11012/244277

BibTex

@article{BUT185011,
  author="Renata {Uhlířová} and Denisa {Langová} and Agáta {Bendová} and Michal {Gross} and Petra {Skoumalová} and Ivana {Márová}",
  title="Antimicrobial Activity of Gelatin Nanofibers Enriched by Essential Oils against Cutibacterium acnes and Staphylococcus epidermidis",
  journal="Nanomaterials",
  year="2023",
  volume="13",
  number="5",
  pages="1--18",
  doi="10.3390/nano13050844",
  issn="2079-4991",
  url="https://www.mdpi.com/2079-4991/13/5/844"
}