Publication detail

Dual mode of IFI16 binding to supercoiled and linear DNA: A closer insight

VALKOVÁ, N. KRATOCHVILOVÁ, L. MARTINKOVÁ, L. BRÁZDA, V.

Original Title

Dual mode of IFI16 binding to supercoiled and linear DNA: A closer insight

Type

journal article in Web of Science

Language

English

Original Abstract

IFI16 (Interferon inducible protein 16) is a DNA sensor responsible for innate immune response stimu-lation and a direct viral restriction by modulating gene expression and replication. Many IFI16-DNA binding properties were described -length-dependent and sequence-independent binding, oligomeri-zation of IFI16 upon recognition, sliding on the DNA, and preference for supercoiled DNA. However, the question of the role of IFI16-DNA binding in distinct IFI16 functions remains unclear. Here we demon-strate two modes of IFI16 binding to DNA using atomic force microscopy and electrophoretic mobility shift assays. In our study, we show that IFI16 can bind to DNA in the form of globular complexes or oligomers depending on DNA topology and molar ratios. The stability of the complexes is different in higher salt concentrations. In addition, we observed no preferential binding with the HIN-A or HIN-B domains to supercoiled DNA, revealing the importance of the whole protein for this specificity. These results provide more profound insight into IFI16-DNA interactions and may be important in answering the question of self-and non-self-DNA binding by the IFI16 protein and potentially could shed light on the role of DNA binding in distinct IFI16 functions.(c) 2023 Elsevier Inc. All rights reserved.

Keywords

IFI16; Superhelicity; DNA; AFM; G-quadruplex; Inverted repeat

Authors

VALKOVÁ, N.; KRATOCHVILOVÁ, L.; MARTINKOVÁ, L.; BRÁZDA, V.

Released

30. 7. 2023

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE

Location

SAN DIEGO

ISBN

0006-291X

Periodical

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Year of study

667

Number

30.07.2023

State

United States of America

Pages from

89

Pages to

94

Pages count

6

URL

https://www.sciencedirect.com/science/article/pii/S0006291X23006125?via%3Dihub

BibTex

@article{BUT185394,
  author="VALKOVÁ, N. and KRATOCHVILOVÁ, L. and MARTINKOVÁ, L. and BRÁZDA, V.",
  title="Dual mode of IFI16 binding to supercoiled and linear DNA: A closer insight",
  journal="BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS",
  year="2023",
  volume="667",
  number="30.07.2023",
  pages="89--94",
  doi="10.1016/j.bbrc.2023.05.049",
  issn="0006-291X",
  url="https://www.sciencedirect.com/science/article/pii/S0006291X23006125?via%3Dihub"
}