Publication detail

Genosensing on a 3D-printed nanocarbon electrode

JYOTI, J. FOJTA, M. HERMANOVÁ, M. PIVOŇKOVÁ, H. ALDUHAISH, O. PUMERA, M.

Original Title

Genosensing on a 3D-printed nanocarbon electrode

Type

journal article in Web of Science

Language

English

Original Abstract

In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.

Keywords

3D-printed nanocarbon electrode; DNA hybridization; Electrochemical analysis; Additive manufacturing

Authors

JYOTI, J.; FOJTA, M.; HERMANOVÁ, M.; PIVOŇKOVÁ, H.; ALDUHAISH, O.; PUMERA, M.

Released

1. 6. 2023

Publisher

Elsevier

Location

NEW YORK

ISBN

1873-1902

Periodical

ELECTROCHEMISTRY COMMUNICATIONS

Year of study

151

Number

1

State

United States of America

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S1388248123000826

Full text in the Digital Library

http://hdl.handle.net/11012/244975

BibTex

@article{BUT186966,
  author="Jyoti {Jyoti} and Miroslav {Fojta} and Monika {Hermanová} and Hana {Pivoňková} and Osamah {Alduhaish} and Martin {Pumera}",
  title="Genosensing on a 3D-printed nanocarbon electrode",
  journal="ELECTROCHEMISTRY COMMUNICATIONS",
  year="2023",
  volume="151",
  number="1",
  pages="8",
  doi="10.1016/j.elecom.2023.107508",
  issn="1873-1902",
  url="https://www.sciencedirect.com/science/article/pii/S1388248123000826"
}