Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes

Development of a Multilayered 4th Generation Scaffold for Advanced Total Skin Substitutes

abstract

Czech

The skin, the body‘s largest organ, serves as a vital barrier protecting against external elements and pathogens. Comprising the epidermis, dermis, and hypodermis, it plays a crucial role in protecting the body. This study focuses on developing a multilayered 4th generation scaffold. The epidermal layer was fabricated using elatin/polycaprolactone/oxidized cellulose nanofibers, while the dermis and hypodermis were formed from a 3D porous scaffold made of collagen type I and antimicrobial chitosan, differing only in scaffold thickness. These layers were crosslinked and characterized using various physicochemical methods, including scanning electron microscopy to assess morphology and pore size. Subsequently, the dermis and hypodermis-mimicking scaffolds were seeded with differentiated mesenchymal stem cells into fibroblasts and preadipocytes. Isolated keratinocytes were then applied atop the fibrous layer to mimic the epidermis. The initial successful in vivo testing of these skin substitutes carried out in a pig model represents a significant step towards their potential clinical application.

The skin, the body‘s largest organ, serves as a vital barrier protecting against external elements and pathogens. Comprising the epidermis, dermis, and hypodermis, it plays a crucial role in protecting the body. This study focuses on developing a multilayered 4th generation scaffold. The epidermal layer was fabricated using elatin/polycaprolactone/oxidized cellulose nanofibers, while the dermis and hypodermis were formed from a 3D porous scaffold made of collagen type I and antimicrobial chitosan, differing only in scaffold thickness. These layers were crosslinked and characterized using various physicochemical methods, including scanning electron microscopy to assess morphology and pore size. Subsequently, the dermis and hypodermis-mimicking scaffolds were seeded with differentiated mesenchymal stem cells into fibroblasts and preadipocytes. Isolated keratinocytes were then applied atop the fibrous layer to mimic the epidermis. The initial successful in vivo testing of these skin substitutes carried out in a pig model represents a significant step towards their potential clinical application.

bilayer scaffold, skin, nanofibers, skin substitutes

bilayer scaffold, skin, nanofibers, skin substitutes

PAVLIŇÁKOVÁ, V.; IZSÁK, D.; LIPOVÝ, B.; KNOZ, M.; SMOLNICKÁ, A.; FALDYNA, M.; JEKLOVÁ, E.; KOUTNÁ, I.; VOJTOVÁ, L.

25. 4. 2024

Praha

978-80-01-07293-6

Bioimplantologie a regenerativní medicína 2024

1. elektronické

43

43

59

https://921c88450d.clvaw-cdnwnd.com/17e12706bb26b88046060a8921d16f31/200000499-73ed673ed8/bioimplantologie_2024_sbornik.pdf?ph=921c88450d