Publication detail

An integrated microfluidic platform for nucleic acid testing

SUN, A. VOPAŘILOVÁ, P. LIU, X. KOU, B. ŘEZNÍČEK, T. LEDNICKÝ, T. NI, S. KUDR, J. ZÍTKA, O. FOHLEROVÁ, Z. PAJER, P. ZHANG, H. NEUŽIL, P.

Original Title

An integrated microfluidic platform for nucleic acid testing

Type

journal article in Web of Science

Language

English

Original Abstract

This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The system integrates the extraction and purification of nucleic acids, followed by amplification via either reverse transcription-quantitative polymerase chain reaction (RT–qPCR) or reverse transcription loop-mediated isothermal amplification (RT–LAMP). By meeting diverse diagnostic and reagent needs, the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR systems. Notable advantages of our system include its speed and cost-effectiveness. The assay is completed within 28 min, including sample loading (5 min), ribonucleic acid (RNA) extraction (3 min), and RT-LAMP (20 min). The cost of each assay is ≈ $9.5, and this pricing is competitive against that of Food and Drug Administration (FDA)-approved commercial alternatives. Although some RNA loss during on-chip extraction is observed, the platform maintains a potential limit of detection lower than 297 copies. Portability makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently located. Another key feature is the platform’s versatility, allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.

Keywords

microfluidics;nucleic acids;RT-PCR;SARS-CoV-2

Authors

SUN, A.; VOPAŘILOVÁ, P.; LIU, X.; KOU, B.; ŘEZNÍČEK, T.; LEDNICKÝ, T.; NI, S.; KUDR, J.; ZÍTKA, O.; FOHLEROVÁ, Z.; PAJER, P.; ZHANG, H.; NEUŽIL, P.

Released

23. 5. 2024

Publisher

Nature

ISBN

2055-7434

Periodical

Microsystems & Nanoengineering

Year of study

10

Number

1

State

People's Republic of China

Pages from

1

Pages to

14

Pages count

14

URL

https://www.nature.com/articles/s41378-024-00677-6

Full text in the Digital Library

http://hdl.handle.net/11012/249356

BibTex

@article{BUT188684,
  author="Antao {Sun} and Petra {Vopařilová} and Xiaocheng {Liu} and Bingqian {Kou} and Tomáš {Řezníček} and Tomáš {Lednický} and Sheng {Ni} and Jiří {Kudr} and Ondřej {Zítka} and Zdenka {Fohlerová} and Petr {Pajer} and Haoqing {Zhang} and Pavel {Neužil}",
  title="An integrated microfluidic platform for nucleic acid testing",
  journal="Microsystems & Nanoengineering",
  year="2024",
  volume="10",
  number="1",
  pages="1--14",
  doi="10.1038/s41378-024-00677-6",
  issn="2055-7434",
  url="https://www.nature.com/articles/s41378-024-00677-6"
}