Publication detail

Homogeneous films from amphiphilic hyaluronan and their characterization by confocal microscopy and nanoindentation

MRÁZEK, J. SITA, J. ONDREÁŠ, F. ZUBOV, A. CHMELAŘ, J.

Original Title

Homogeneous films from amphiphilic hyaluronan and their characterization by confocal microscopy and nanoindentation

Type

journal article in Web of Science

Language

English

Original Abstract

Self-supporting films from amphiphilic hyaluronan are suitable for medical applications like wound dressings or resorbable implants. These films are typically cast from water/alcohol solutions. However, when the mixed solvent evaporates in ambient air, convection flows develop in the solution and become imprinted in the film, potentially compromising its properties. Consequently, we developed a novel film manufacturing method: drying in a closed box under saturated vapour conditions. Using this approach, we prepared a series of optically clear lauroyl-hyaluronan (LHA) films with uniform thickness and compared them to their air-dried counterparts. We first evaluated swelling ratios and elastic moduli for LHA films with varying degrees of substitution. The boxdried films swelled significantly less and were 1-2 orders of magnitude stiffer than air-dried films from the same LHA sample. Confocal microscopy revealed that box-dried films exhibited a regular microstructure, while air-dried films displayed a pore-size gradient and strong microstructure modulation due to convection flows. Local elastic modulus variations arising from these microstructures were assessed using nanoindentation mapping. Importantly, achieving the desired film stiffness requires much lower polymer modification when boxdrying is used, enhancing the biological response to the material. These findings have implications for all polysaccharide formulations that utilize mixed solvents.

Keywords

Hyaluronan; Film; Hydrogel; Solution casting; Mixed solvent; Microstructure; Nanoindentation

Authors

MRÁZEK, J.; SITA, J.; ONDREÁŠ, F.; ZUBOV, A.; CHMELAŘ, J.

Released

27. 5. 2024

Publisher

ELSEVIER SCI LTD

Location

London

ISBN

0144-8617

Periodical

Carbohydrate Polymers

Year of study

340

Number

122331

State

United Kingdom of Great Britain and Northern Ireland

Pages count

10

URL

https://www.sciencedirect.com/science/article/pii/S0144861724005575?via=ihub

BibTex

@article{BUT189030,
  author="MRÁZEK, J. and SITA, J. and ONDREÁŠ, F. and ZUBOV, A. and CHMELAŘ, J.",
  title="Homogeneous films from amphiphilic hyaluronan and their characterization by confocal microscopy and nanoindentation",
  journal="Carbohydrate Polymers",
  year="2024",
  volume="340",
  number="122331",
  pages="10",
  doi="10.1016/j.carbpol.2024.122331",
  issn="0144-8617",
  url="https://www.sciencedirect.com/science/article/pii/S0144861724005575?via=ihub"
}