Publication detail

Advanced 3D collagen-based scaffolds enriched with vasculogenic compound for enhanced vascularization and wound healing

IZSÁK, D. PAVLIŇÁKOVÁ, V. FOHLEROVÁ, Z. SZOTKOWSKÁ, T. BUCHTOVÁ, M. VOJTOVÁ, L.

Original Title

Advanced 3D collagen-based scaffolds enriched with vasculogenic compound for enhanced vascularization and wound healing

Type

abstract

Language

English

Original Abstract

Skin serves as a protective barrier for the human body against toxins and microbes in the environment. The presence of biomaterials at a wound site plays a crucial role in influencing the production of the skin's extracellular matrix (ECM). This research focuses on protein-based biomaterials designed to mimic the ECM and regulate biomechanical processes at the cellular level, including cell migration, proliferation, and differentiation. In this study, porous 3D collagen scaffolds were prepared by using the freeze-drying method. To investigate the effect of additives, the scaffolds were modified with chitosan followed by crosslinking using carbodiimide crosslinking agents. Subsequently, varying concentrations of a pro-angiogenic bioactive substance were incorporated into the scaffolds to enhance the production of vascular endothelial growth factor in fibroblast cells and to improve dermal wound healing. Physicochemical characterization of the prepared carriers involved techniques such as scanning electron microscopy to assess morphology and pore size. The release of pro-angiogenic molecules from the scaffolds was monitored through UV-VIS analysis. Furthermore, cytotoxicity assessments were conducted in-vitro using mouse fibroblasts, while neovascularization studies were performed in ovo using chicken embryos. This comprehensive approach not only elucidates the potential of protein-based biomaterials in wound healing but also highlights the importance of tailored scaffold design for promoting effective tissue regeneration. This work was supported by the CEITEC BUT Specific Research project no. CEITEC VUT-J-24-8629, by the European Fund for Regional Development within project no. CZ.02.01.01/00/22_008/0004562 and by the Ministry of Health of the Czech Republic under project No. NU22-08–00454 as well.

Keywords

Wound healing, vascularization, scaffolds, collagen, chitosan, DMOG

Authors

IZSÁK, D.; PAVLIŇÁKOVÁ, V.; FOHLEROVÁ, Z.; SZOTKOWSKÁ, T.; BUCHTOVÁ, M.; VOJTOVÁ, L.

Released

2. 7. 2024

Location

Thessaloniki, Greece

Pages from

171

Pages to

171

Pages count

220

URL

https://www.nanotexnology.com/index.php/abstract-books-2024/send/5-abstract-books-2024/16-nn24-abstract-book

BibTex

@misc{BUT189360,
  author="Dávid {Izsák} and Veronika {Pavliňáková} and Zdenka {Fohlerová} and Tereza {Szotkowská} and Marcela {Buchtová} and Lucy {Vojtová}",
  title="Advanced 3D collagen-based scaffolds enriched with vasculogenic compound for enhanced vascularization and wound healing",
  booktitle="21st International Conference on Nanosciences & Nanotechnologies, Book of Abstract, Greece, Thessaloniki, 2-5 July 2024",
  year="2024",
  pages="171--171",
  address="Thessaloniki, Greece",
  url="https://www.nanotexnology.com/index.php/abstract-books-2024/send/5-abstract-books-2024/16-nn24-abstract-book",
  note="abstract"
}