Publication detail

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

NYKRÝNOVÁ, M. BEZDÍČEK, M. LENGEROVÁ, M. VÍTKOVÁ, H.

Original Title

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

Type

conference paper

Language

English

Original Abstract

In recent years, sequencing has become easily accessible and widespread, revolutionizing our ability to study complex genetic information. Thus, it is possible to analyze complex bacterial populations and investigate relationships between individual strains. Methods such as core genome multilocus sequence typing (cgMLST) are used in routine clinical practice to characterize bacterial isolates, track the spread of infectious diseases, and monitor outbreaks. However, the limitation lies in distinguishing closely related bacterial strains, which differ only in several bases. Therefore, here, we present the core genome methylome analysis utilizing nanopore sequencing. We demonstrate that epigenetic information contained within bacterial strains allows us to differentiate populations similarly to cgMLST. Moreover, the proposed unique combination of cgMLST with core genome methylome can even increase the discriminatory power between closely similar isolates, overcoming the constraints of cgMLST. Combining genomic and epigenomic information can provide better insight into bacterial strains’ evolution, transmission patterns and pathogenicity study.

Keywords

methylome; cgMLST; bacteria; resistance

Authors

NYKRÝNOVÁ, M.; BEZDÍČEK, M.; LENGEROVÁ, M.; VÍTKOVÁ, H.

Released

28. 8. 2024

Publisher

Springer Nature

ISBN

978-3-031-64636-2

Book

Lecture Notes in Bioinformatics

Edition

14849

Edition number

II.

ISBN

1611-3349

Periodical

Lecture Notes in Computer Science

Year of study

14849

Number

II.

State

Republic of Italy

Pages from

140

Pages to

152

Pages count

13

URL

https://link.springer.com/chapter/10.1007/978-3-031-64636-2_11

BibTex

@inproceedings{BUT189432,
  author="Markéta {Jakubíčková} and Matěj {Bezdíček} and Martina {Lengerová} and Helena {Vítková}",
  title="Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae",
  booktitle="Lecture Notes in Bioinformatics",
  year="2024",
  series="14849",
  journal="Lecture Notes in Computer Science",
  volume="14849",
  number="II.",
  pages="140--152",
  publisher="Springer Nature",
  doi="10.1007/978-3-031-64636-2\{_}11",
  isbn="978-3-031-64636-2",
  issn="1611-3349",
  url="https://link.springer.com/chapter/10.1007/978-3-031-64636-2_11"
}