Publication detail

Inertial co-focusing of heterogeneous particles in hybrid microfluidic channels with constantly variable cross-sections

ZAHO, T. ZHANG, Y. LI, J. SUN, H. GABLECH, I. CHANG, H. YUAN, X. NEUŽIL, P. FENG, J.

Original Title

Inertial co-focusing of heterogeneous particles in hybrid microfluidic channels with constantly variable cross-sections

Type

journal article in Web of Science

Language

English

Original Abstract

Heterogeneous particles co-focusing to a single stream is a vital prerequisite for cell counting and enumeration, playing an essential role in flow cytometry and single-cell analysis. Microfluidics-based inertial focusing holds great research prospects due to its simplicity of devices, ease of operation, high throughput, and freedom from external fields. Combining microfluidic channels with two or more different geometries has become a powerful tool for high-efficiency particle focusing. Here, we explored hybrid microfluidic channels for heterogeneous particle co-focusing. Four different annular channels with obstacles distributed on the inner wall were constructed and simulated, obtaining constantly variable secondary flows. Then we used four different fluorescent particles with the size of 10 mu m, 12 mu m 15 mu m, and 20 mu m as well as their mixture to perform the inertial focusing experiments of multi-sized particles. Theoretical simulation and experimental results demonstrated a focusing efficiency of >99%. Finally, we further utilized human white blood cells to estimate the co-focusing performance of our hybrid microfluidic channel, resulting in a high focusing efficiency of >92% and a high throughput of approximate to 8000 cell s(-1). The hybrid microfluidic channels, capable of high-precision heterogeneous particle co-focusing, could pave a broad avenue for microfluidic flow cytometry and single-cell analysis.

Keywords

separation; flow

Authors

ZAHO, T.; ZHANG, Y.; LI, J.; SUN, H.; GABLECH, I.; CHANG, H.; YUAN, X.; NEUŽIL, P.; FENG, J.

Released

30. 9. 2024

Publisher

ROYAL SOC CHEMISTRY

Location

England

ISBN

1473-0189

Periodical

LAB ON A CHIP

Year of study

24

Number

21

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

11

Pages count

11

URL

https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00479e

BibTex

@article{BUT189818,
  author="ZAHO, T. and ZHANG, Y. and LI, J. and SUN, H. and GABLECH, I. and CHANG, H. and YUAN, X. and NEUŽIL, P. and FENG, J.",
  title="Inertial co-focusing of heterogeneous particles in hybrid microfluidic channels with constantly variable cross-sections",
  journal="LAB ON A CHIP",
  year="2024",
  volume="24",
  number="21",
  pages="11",
  doi="10.1039/d4lc00479e",
  issn="1473-0189",
  url="https://pubs.rsc.org/en/content/articlelanding/2024/lc/d4lc00479e"
}