Publication detail

Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

A. M., A. SELCUK, O. MUKHERJEE, A. UNAL, D. KURBANOGLU, S. USLU, B. JUŘICA, J. PEKÁRKOVÁ, J. RICHTERA, L. ADAM, V.

Original Title

Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

Type

journal article in Web of Science

Language

English

Original Abstract

Determination of plasma uracil was reported as a method for evaluation of Dihydropyrimidine dehydrogenase (DPD) activity that is highly demanded to ensure the safe administration of 5-fluorouracil (5-FU)-based therapies to cancer patients. This work reports the development of a simple electroanalytical method based on adsorptive stripping square wave voltammetry (AdSWV) at mercury film-coated glassy carbon electrode (MF/GCE) for the highly sensitive determination of uracil in biological fluids that can be used for diagnosis of decreased DPD activity. Due to the formation of the HgII-Uracil complex at the electrode surface, the accuracy of the measurement was not affected by the complicated matrices in biological fluids including human serum, plasma, and urine. The high sensitivity of the developed method results in a low limit of detection (approximate to 1.3 nM) in human plasma samples, falling below the practical cut-off level of 15 ng mL-1 (approximate to 0.14 mu M). This threshold concentration is crucial for predicting 5-FU toxicity, as reported in buffer, and <= 1.15% in biological samples), and accuracy (recovery percentage close to 100%).

Keywords

Mercury thin film electrode; Adsorptive stripping voltammetry; Uracil determination; Dihydropyrimidine dehydrogenase; phenotyping; 5-Fluorouracil-based therapies

Authors

A. M., A.; SELCUK, O.; MUKHERJEE, A.; UNAL, D.; KURBANOGLU, S.; USLU, B.; JUŘICA, J.; PEKÁRKOVÁ, J.; RICHTERA, L.; ADAM, V.

Released

28. 6. 2024

Publisher

ELSEVIER ADVANCED TECHNOLOGY

Location

OXFORD

ISBN

1873-4235

Periodical

BIOSENSORS & BIOELECTRONICS

Year of study

262

Number

116545

State

Kingdom of the Netherlands

Pages from

1

Pages to

7

Pages count

7

URL

https://www.sciencedirect.com/science/article/pii/S0956566324005505?via%3Dihub

BibTex

@article{BUT189918,
  author="Ashrafi {A. M.} and Ozge {Selcuk} and Atripan {Mukherjee} and Didem Nur {Unal} and Sevinc {Kurbanoglu} and Bengi {Uslu} and Jan {Juřica} and Jana {Pekárková} and Lukáš {Richtera} and Vojtěch {Adam}",
  title="Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2024",
  volume="262",
  number="116545",
  pages="7",
  doi="10.1016/j.bios.2024.116545",
  issn="1873-4235",
  url="https://www.sciencedirect.com/science/article/pii/S0956566324005505?via%3Dihub"
}