Publication detail

A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cells

FLEISCHHACKER-DAFFERT, C. ZEROBIN, A. HUMMEL, F. SLANINOVÁ, E. KROUPOVÁ, Z. OBRUČA, S. MRÁZOVÁ, K. HRUBANOVÁ, K. KRZYŽÁNEK, V. NEBESÁŘOVÁ, J. LUDWIG, K. FRITZ, I.

Original Title

A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cells

Type

journal article in Web of Science

Language

English

Original Abstract

Poly(3-hydroxybutyrate) (PHB) is a biobased and biodegradable polymer with properties comparable to polypropylene and therefore has the potential to replace conventional plastics. PHB is intracellularly accumulated by prokaryotic organisms. For the cells PHB functions manly as carbon and energy source, but all possible functions of PHB are still not known. Synechocystis (cyanobacteria) accumulates PHB using light as energy and CO2 as carbon source. The main trigger for PHB accumulation in cyanobacteria is nitrogen and phosphorous depletion with simultaneous surplus of carbon and energy. For the above reasons, obtaining knowledge about external factors influencing PHB accumulation is of highest interest. This study compares the effect of continuous light exposure and day/night (16/8 h) cycles on selected physiology parameters of three Synechocystis strains. We show that continuous illumination at moderate light intensities leads to an increased PHB accumulation in Synechocystis salina CCALA 192 (max. 14.2% CDW - cell dry weight) compared to day/night cycles (3.7% CDW). In addition to PHB content, glycogen and cell size increased, while cell density and cell viability decreased. The results offer new approaches for further studies to gain deeper insights into the role of PHB in cyanobacteria to obtain bioplastics in a more sustainable and environmentally friendly way.

Keywords

Synechocystis; continuous illumination; day/night cycle; PHB; glycogen; cell size

Authors

FLEISCHHACKER-DAFFERT, C.; ZEROBIN, A.; HUMMEL, F.; SLANINOVÁ, E.; KROUPOVÁ, Z.; OBRUČA, S.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; KRZYŽÁNEK, V.; NEBESÁŘOVÁ, J.; LUDWIG, K.; FRITZ, I.

Released

20. 7. 2024

Publisher

MDPI

Location

BASEL

ISBN

2075-1729

Periodical

Life

Year of study

14

Number

7

State

Swiss Confederation

Pages count

18

URL

https://www.mdpi.com/2075-1729/14/7/907

BibTex

@article{BUT191234,
  author="Christina {Fleischhacker-Daffert} and Antonia {Zerobin} and Ferdinand {Hummel} and Eva {Slaninová} and Zuzana {Kroupová} and Stanislav {Obruča} and Kateřina {Mrázová} and Kamila {Hrubanová} and Vladislav {Krzyžánek} and Jana {Nebesářová} and Katharina {Ludwig} and Ines {Fritz}",
  title="A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation in Synechocystis Cells",
  journal="Life",
  year="2024",
  volume="14",
  number="7",
  pages="18",
  doi="10.3390/life14070907",
  issn="2075-1729",
  url="https://www.mdpi.com/2075-1729/14/7/907"
}