Publication detail

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

RIENMÜLLER, T. SHRESTHA, N. POLZ, M. STOPPACHER, S. ZIESEL, D. MIGLIACCIO, L. PELZMANN, B. LANG, P. ZORN-PAULY, K. LANGTHALER, S. OPANČAR, A. BAUMGARTNER, C. ÜÇAL, M. SCHINDL, R. DEREK, V. SCHERUEBEL, S.

Original Title

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Type

journal article in Web of Science

Language

English

Original Abstract

Objective: This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltage-gated Cav1.2 channels (I-Ca,I-L) in Guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated I-Ca,I-L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated I-Ca,I-L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca2+ channel blocker, nifedipine. Light-triggered I-Ca,I-L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of I-Ca,I-L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of I-Ca,I-L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimal-invasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.

Keywords

Biomedical modeling and simulation; calcium; cardiac physiology; electrophysiology; optoelectronic devices; patch-clamp; voltage-gated ion channels

Authors

RIENMÜLLER, T.; SHRESTHA, N.; POLZ, M.; STOPPACHER, S.; ZIESEL, D.; MIGLIACCIO, L.; PELZMANN, B.; LANG, P.; ZORN-PAULY, K.; LANGTHALER, S.; OPANČAR, A.; BAUMGARTNER, C.; ÜÇAL, M.; SCHINDL, R.; DEREK, V.; SCHERUEBEL, S.

Released

3. 6. 2024

Publisher

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

Location

PISCATAWAY

ISBN

0018-9294

Periodical

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING

Year of study

71

Number

6

State

United States of America

Pages from

1980

Pages to

1992

Pages count

13

URL

https://ieeexplore.ieee.org/document/10473191

BibTex

@article{BUT197349,
  author="Theresa {Rienmüller} and Niroj {Shrestha} and Mathias {Polz} and Sara {Stoppacher} and Daniel {Ziesel} and Ludovico {Migliaccio} and Brigitte {Pelzmann} and Petra {Lang} and Klaus {Zorn-Pauly} and Sonja {Langthaler} and Aleksandar {Opančar} and Christian {Baumgartner} and Muammer {Üçal} and Rainer {Schindl} and Vedran {Derek} and Susanne {Scheruebel}",
  title="Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices",
  journal="IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING",
  year="2024",
  volume="71",
  number="6",
  pages="1980--1992",
  doi="10.1109/TBME.2024.3358240",
  issn="0018-9294",
  url="https://ieeexplore.ieee.org/document/10473191"
}