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REALE, L. KAISER, J. REALE, A. LAI, A. FLORA, F. BALERNA, A. CINQUE, G. FANELLI, M. RUGGIERI, F. FAENOV, A. PIKUZ, T. TUCCI, A. POMA, A. ZUPELLA, P. LIŠKA, M. MALINA, R.
Original Title
Mapping the intake of different elements in vegetal tissues by dual-energy X-ray imaging at DaFne synchrotron light source
Type
journal article - other
Language
English
Original Abstract
This article reports on the first utilization of the soft X-ray beamline at the DaFne synchrotron light source for mapping the intake of different elements in plant tissues. As a test, the method of dual-energy X-ray microradiography was applied to the investigation of the natural sulfur content in dried leaf and root samples. Our ultimate goal was to monitor the pollutant lead and its intake, which was added in controlled doses to the hydroponic medium of laboratory-controlled samples of vegetal species. The results obtained by the nondestructive X-ray radiographic analysis are compared to the values of concentrations determined by a standard chemical analysis utilizing atomic absorption spectroscopy. From this comparison the validity of the X-ray detection of heavy metals in biological samples has been confirmed. The superposition of the dual energy results on the simple planar radiography shows the representation of the pollutant intake directly on the sample structures. It should be pointed out that this method, developed here for plant root and leaves could be applied to any biological sample of interest, but the preparation and observation conditions necessitate different strategies according to the type of sample under analysis.
Keywords
dual energy X-ray analysis, intake, biodisposable, sulphur, lead
Authors
REALE, L.; KAISER, J.; REALE, A.; LAI, A.; FLORA, F.; BALERNA, A.; CINQUE, G.; FANELLI, M.; RUGGIERI, F.; FAENOV, A.; PIKUZ, T.; TUCCI, A.; POMA, A.; ZUPELLA, P.; LIŠKA, M.; MALINA, R.
RIV year
2008
Released
3. 3. 2008
ISBN
1059-910X
Periodical
Microscopy research and technique
Year of study
71
Number
3
State
United States of America
Pages from
179
Pages to
185
Pages count
7
BibTex
@article{BUT45022, author="REALE, L. and KAISER, J. and REALE, A. and LAI, A. and FLORA, F. and BALERNA, A. and CINQUE, G. and FANELLI, M. and RUGGIERI, F. and FAENOV, A. and PIKUZ, T. and TUCCI, A. and POMA, A. and ZUPELLA, P. and LIŠKA, M. and MALINA, R.", title="Mapping the intake of different elements in vegetal tissues by dual-energy X-ray imaging at DaFne synchrotron light source", journal="Microscopy research and technique", year="2008", volume="71", number="3", pages="179--185", issn="1059-910X" }