Detection of lead in Zea mays by dual-energy X-ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

journal article - other

English

This study is related to the application of the X-ray dual-energy microradiography technique together with the atomic absorption spectroscopy (AAS) for the detection of lead on Zea mays stem, ear, root, and leaf samples. To highlight the places with lead intake, the planar radiographs taken with monochromatic X-ray radiation in absorption regime with photon energy below and above the absorption edge of a given chemical element, respectively, are analyzed and processed. To recognize the biological structures involved in the intake, the dual-energy images with the lead signal have been compared with the optical images of the same Z. mays stem. The ear, stem, root, and leaf samples have also been analyzed with the AAS technique to measure the exact amount of the hyperaccumulated lead. The AAS measurement revealed that the highest intake occurred in the roots while the lowest in the maize ears and in the leaf. It seems there is a particular mechanism that protects the seeds and the leaves in the intake process.

dual-energy X-ray analysis, intake, biodisposable, Zea mays, stem, lead

REALE, L. -KAISER, J. -PACE, L. -LAI, A. -FLORA, F. -BRUNO, A. -TUCCI, A. -ZUPPELLA, P. -MANCINI, L. -TROMBA, G. -RUGGIERI, F. -FANELLI, M. -MALINA, R. -LIŠKA, M. -POMA, A.

2009

1. 12. 2009

1059-910X

Microscopy research and technique

XXX

X

United States of America

na

na

12