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TRACHTOVÁ, Š. ŠPANOVÁ, A. RITTICH, B. HORÁK, D.
Original Title
Characterization of magnetic and non-magnetic solid polymer microspheres
Type
abstract
Language
English
Original Abstract
Magnetic separation is one of modern methods to speed up and facilitate the previously used separation and purification procedures. The aim of this work was to study the potential inhibition effect of different types of commercial and non-commercial magnetic and non-magnetic carriers on PCR course. It was shown that most of carriers does not influence the course of PCR. In second part, reversible DNA adsorption on surface of magnetic particles was tested in the presence of an aqueous phase system containing 16 % PEG 6000 and 2 M NaCl from complex samples, namely dairy products.
Keywords
magnetic- and non-magnetic particles, PCR, real time PCR, PCR inhibiton, dairy products
Authors
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.; HORÁK, D.
Released
17. 8. 2011
Publisher
česká společnost chemická
Location
Praha, ČR
ISBN
0009-2770
Periodical
Chemické listy
Year of study
105
Number
18
State
Czech Republic
Pages from
s988
Pages to
s989
Pages count
2