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TRACHTOVA, S. OBERMAJER, T. SPANOVA, A. BOGOVIC MATIJASIC, B. ROGELJ, I. HORAK, D. RITTICH, B.
Original Title
Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces
Type
journal article in Web of Science
Language
English
Original Abstract
Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of target DNA were verified by PCR and real time PCR using primers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxyl-coated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR.
Keywords
magnetic microspheres; c); DNA isolation; mouse faeces; polymerase chain reaction (PCR)
Authors
TRACHTOVA, S.; OBERMAJER, T.; SPANOVA, A.; BOGOVIC MATIJASIC, B.; ROGELJ, I.; HORAK, D.; RITTICH, B.
RIV year
2012
Released
15. 2. 2012
Publisher
Taylor & Francis
Location
Londýn, Spojené království Velké Británie a Severního Irska
ISBN
1542-1406
Periodical
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
Year of study
555
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
263
Pages to
270
Pages count
7
BibTex
@article{BUT73651, author="Štěpánka {Trachtová} and Tanja {Obermajer} and Alena {Španová} and Bojana {Bogovič Matijašić} and Irena {Rogelj} and Daniel {Horák} and Bohuslav {Rittich}", title="Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for DNA isolation from faeces", journal="MOLECULAR CRYSTALS AND LIQUID CRYSTALS", year="2012", volume="555", number="1", pages="263--270", doi="10.1080/15421406.2012.635554", issn="1542-1406" }