Course detail

Gene Technology

FCH-BCO_GETAcad. year: 2011/2012

Basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC. Identification of recombinant DNA. Gene expression in microbial (bacteria, yeast) and animal expression systems, markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection).

Language of instruction

Czech

Number of ECTS credits

3

Mode of study

Not applicable.

Guarantor

prof. RNDr. Ivana Márová, CSc.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Fundamental principles and theoretical knowledge of gene technologies and their biotechnological applications. New strategies in genetic engineering.

Prerequisites

Examination - Biochemistry I. Basic knowledge about structure and function of biomolecules.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

Examination - written and oral part. Elaboration and prezentation of seminar project.

Course curriculum

1. Basic terms of molecular genetics; structure and function of information macromolecules
2. Overview of methods for isolation and analysis of nucleic acids
3. Transformation vectors, restriction endonucleases and other enzymes used in recombinant DNA technology
4. Hybridizationof nucleic acids.
5. Methods of transformation, criteria for recombinant cell selection.
6. Clonning of DNA, polymerase chain reaction and its applications in clinical and food diagnostics.
7. Molecular analysis of genome - sequenation. Gene libraries.
8. Mutagenesis.
9. Production, isolation and analysis of recombinant proteins.
10. Construction of transgenic organisms, optimalization of transgene expression.
11. Genetically modified microoorganisms and their applications.
12. Transformation of higher organisms - plants, animals.
13. Overview of applications of genetic engineering in food processing and biotechnology, gene therapy.

Work placements

Not applicable.

Aims

This course is focused on introduction of basic methods and techniques used in gene technology and recombinant DNA technology: isolation, characterization and analysis of nucleic acids, DNA clonning, polymerase chain reaction (PCR), gene mapping, methods of transformation, types of transformation vectors, identification of recombinant DNA, foreign gene expression in prokaryotic and eukaryotic expression systems, identification and purification of expressed recombinant proteins.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not compulsory, but is recommended. Attendance at seminars is compulsory and controlled by lecturer.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

/e-learning FCH (CS)
Rosypal S.: Úvod do molekulární biologie. Brno, 2002 (CS)

Recommended reading

Alberts a kol. : Základy buněčné biologie. Espero Publishong 2001. (CS)

Classification of course in study plans

  • Programme BPCP_CHTP Bachelor's

    branch BPCO_BT , 3 year of study, summer semester, compulsory

  • Programme BKCP_CHTP Bachelor's

    branch BKCO_BT , 3 year of study, summer semester, compulsory

Type of course unit

 

Seminar

13 hod., compulsory

Teacher / Lecturer

prof. RNDr. Ivana Márová, CSc.

Lecture

26 hod., optionally

Teacher / Lecturer

prof. RNDr. Ivana Márová, CSc.

Guided consultation in combined form of studies

39 hod., optionally

Teacher / Lecturer

prof. RNDr. Ivana Márová, CSc.