Physical bases of mass spectrometry, MS history. Ionization techniques - listing, development, description of contemporary used techniquies (electron ionization, chemical ionization, electrospray, chemical ionization at atmospheric pressure etc). Analyzers in mass spectrometry (static - magnetic, elctrostatic, quadrupole, 3D- and linear ion trap, Time-of-Flight, ion cyclotron resonance, orbitrap). Detectors and vacuum systems. Hyphenated and tandm techniquies. Interpretation of mass spectra with respect to the ionization technique applied.