Course detail

Molecular genetics II

FCH-MCO_MGE2Acad. year: 2011/2012

Molecular mechanisms of mutagenesis and recombination. Mobile elements. Repair mechanisms of damaged DNA. Basic methods of molecular genetics. DNA cloning. Gene engineering and transgenic organisms. Gene therapy. Molecular genetics of human. Genetic diseases and their diagnistics. Molecular genetics of plants and their use in agriculture.

Language of instruction

Czech

Number of ECTS credits

2

Mode of study

Not applicable.

Guarantor

prof. RNDr. Jiří Doškař, CSc.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Basic knowledge of the methods of molecular biology (DNA cloning, detection of transgenes, preparation of transgenic organisms). Characteristics of transgenic bacteria, yerast, plants and animals, and their use in medicine, agriculture and industry. Preparation of biotechnologically important substances by the methods of gene engineering.

Prerequisites

The structure and basic functions of living systems, their reproduction, cell chemistry, structure and function of biomacromolecules.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

During the examination the student answers questions in writen form concerning subject matter of the teaching courses.

Course curriculum

Definition of gene engineering, its history and perspectives , Mutagenesis in vitro , Protein engineering , Optimalization of gene expression , Gene cloning in bacteria , Gene cloning in plants, gene cloning in animals, Characteristics of cloning vectors, Gene transfer in embryonal cells , Gene therapy, Preparation of pharmacologically important substances in heterologous systems, Risc of manipulations with GMO

Work placements

Not applicable.

Aims

Knowledge about the use of molecular biology methods in gene maniuplations and in preparation of genetically modified organisms. At the end of this course students understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and practical application

Specification of controlled education, way of implementation and compensation for absences

Lectures are optional, no control tests during the semester

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Glick B. R., Pasternak J. J.: Molecular Biotechnology. ASM Press, Washington 2003. (CS)
Primrose S. B., Twyman R. M.: Principles of gene manipulation. Blackwell Publishing, Cornwall 2006. (CS)
Watson, J. D. et al.: Recombinant DNA. Scientific American Books, New York 1992. (CS)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 1 year of study, winter semester, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 1 year of study, winter semester, compulsory-optional

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

prof. RNDr. Jiří Doškař, CSc.

Guided consultation in combined form of studies

13 hod., optionally

Teacher / Lecturer

prof. RNDr. Jiří Doškař, CSc.