Course detail

Light Microscopy

FSI-9MIKAcad. year: 2011/2012

The course explains principles of light microscopy in terms of geometrical and wave optics. Imaging of extended objects is analysed in details using the formalism of pupil and transfer functions. Fundamentals of advanced microscopis methods are explained as well as the directions of their development (confocal microscopy, fluorescence microscopy, interference microscopy, nonlinear phenomena, Raman scattering, stimulated light emission). The lecture is illustrated by the demonstrations and experiments with the light microscopes.

Language of instruction

Czech

Number of ECTS credits

2

Mode of study

Not applicable.

Guarantor

prof. RNDr. Radim Chmelík, Ph.D.

Department

Institute of Physical Engineering (ÚFI)

Learning outcomes of the course unit

Knowledge of fundamentals of microscopy and competence to apply them in practice.

Prerequisites

Not applicable.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course content is explained by means of tutorials completed by practical demonstrations in laboratory od optical microscopy.

Assesment methods and criteria linked to learning outcomes

Examination is oral. Detailed knowledge of explained matter is proved. Examined student can use any literature.

Course curriculum

Not applicable.

Work placements

Not applicable.

Aims

Understanding of principles of light microscopy and microscopic imaging, knowledge of conventional and advanced microscopic techniques.

Specification of controlled education, way of implementation and compensation for absences

Not applicable.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Born, M., Wolf, E.: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999 (EN)
T. Wilson: Confocal Microscopy. Academic Press, London 1990 (EN)

Recommended reading

J. W. Goodman: Introduction to Fourier Optics. 2nd ed.. McGraw-Hill, New York 1996
M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999

Type of course unit

 

Lecture

20 hod., optionally

Teacher / Lecturer

prof. RNDr. Radim Chmelík, Ph.D.

Syllabus

1. Light as the electromagnetic wave, coherence of light, Hopkins' formula.
2. Fundamentals of geometric optics, elementary parts of the microscope.
3. Historical overview.
4. Wave description of imaging, the Abbe theory, the impulse response.
5. Coherent and optical transfer function.
6. Basic techniques of conventional microscopy, bright and dark field.
7. Phase contrast.
7. Polarized light.
8. Confocal microscopy.
9. Interference microscopy.
10. Fluorescence microscopy, modern techniques of microscopy.