Czech

Not applicable.

Graduate obtains knowledge about the theory of chromatographic separation techniques, about the instrumentation of liquid and gas chromatography, about their application in analytical praxis. He/She will get ability to select the most suitable chromatographic technique for the solution of real analytical tass and to critically evaluate obtained data.

Thorough knowledge of physical chemistry, good knowledge of common, inorganic, organic and analytical chemistry.

Not applicable.

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Oral examination covering the range of discussed topics

1. Introduction, history of chromatography, classification of chromatographic techniques
2. Basic theory of chromatography – thermodynamical and kinetic aspects, effectivity of chromatographic separation
3. Planar chromatography
4. Column chromatography – basic configuration and theory
5. High performance liquid chromatography – separation principles, instrumentation, columns and rules of their selection, normal and reversed-phase systems, isocratic and gradient elution, ion chromatography, ion-pair chromatography, hydrophobic interaction liquid chromatography, size exclusion chromatography, bioaffinity chromatography, current trends – UPLC, UHPLC
6. Gas chromatography – separation principles, instrumentation, capillary and packed columns, rules for their selection, classification of stationary phases in gas chromatography, derivatization in gas chromatography
7. Tandem techniques in chromatography – systems LC-LC, LC-GC, GC-GC, GCxGC
8. Qualitative aspects of chromatographic data – correlation structure-retention, retention indices in gas chromatography
9. Quantitation in chromatography

Not applicable.

Thorough familiarization of students with chromatographic separation techniques and with application of chromatography including interpretation of chromatographic data

Not applicable.

Not applicable.

Not applicable.

Churáček J. a kol.: Analytická separace látek. STNL Praha, 1990 (CS)
Churáček J. a kol.: Nové trendy v teorii a instrumentaci vybraných analytických metod. SNTL Praha, 1993. (CS)
V. R. Meyer: Practical High-Performance. Liquid Chromatography, 2004 (EN)

Not applicable.