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FCH-MC_BIO2Acad. year: 2012/2013
Stechiometry of microbial growth and material balances. Systems with immobilized cells and enzymes. Enzymatic catalysis in anhydrous and quasi anhydrous solvents. Magnetic particles. Micromanipulation of particles. Microflow in capillary tubes. Microdrops. Properties of fluids. Mechanical, chemical and enzymatic cell disruption. Precipitation and salting-out odf proteins. Filtration. Centrifugation. Extraction - types of extractors and their design. Extraction using aqueous two-phase systems. Large scale chromatographic techniques. Production of proteins using genetically modified microorganisms (GMO) and methods of their isolation. Storage of proteins. Validation of production quality.
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branch NPCO_CHTP , 1 year of study, winter semester, compulsory
branch NKCO_CHTP , 1 year of study, winter semester, compulsory
branch CKCO_CZV , 1 year of study, winter semester, compulsory
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