Course detail

Basic Principles of Modern Biotechnologies

FCH-BCO_ZMAcad. year: 2012/2013

Students will be introduced to biotechnological processes used for production of selected microbial, mammalian, hybridome and plant substances. Further, principles of fermentation procedures, separation of cells from media, dissintegration processes, separation techniques and purification of biomolecules will be discussed. As a part of this course basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC will be introduced. Methods of identification of recombinant DNA will be explained. Gene expression in microbial (bacteria, yeast) and animal expression systems will be described including expression markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection) will be introduced too.

Language of instruction

Czech

Number of ECTS credits

5

Mode of study

Not applicable.

Guarantor

prof. RNDr. Ivana Márová, CSc.

Department

Materials Research Centre (CMV)

Learning outcomes of the course unit

Principles and theoretical basis of modern biotechnologies, mainly gene technologies and some kinds of their applications. Novel strategies in gene engineering.

Prerequisites

Not applicable.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.
The lectures are focused on explanation of basic principles of gene technologies. If knowledge higher than the basic is required in order to explain a certain effect, the necessary supplementing information (video etc) is included in the lecture so as the discussed topic is understandable. The lectures are accompanied by seminars with individual students presentations, video- demontrations, discussion and many instructive explanations and illustrations of practical applications. If it is possible, the teaching is realized by "problem-based learning" technique.

Assesment methods and criteria linked to learning outcomes

Writing test and oral examination. Elaboration and presentation of seminary project.

Course curriculum

1. Basic terms of molecular genetics; structure and function of information macromolecules.
2. Methods of isolation and analysis of biomolecules
3. Transformation vectors, restriction endonucleases and other enzymes used to analysis and processing of nucleic acids.
4. Hybridization.
5. Methods of transformation, selection criteria of recombninants.
6. Clonning of DNA, polymerase chain reaction, use of PCR to clinical and food diagnostics.
7. Molecular analysis of genome - sequencing. Gene libraries.
8. Mutagenesis - random, site-specific.
9. Production, isolation and analysis of recombinant proteins.
10. Preparation of transgenic organisms, optimization of transgene expression.
11. Genetically modified microorganisms and their applications.
12. Transformation of higher organisms - plant, animals.

Work placements

Not applicable.

Aims

Selection of biological system for production of nucleic acids, proteins and for gene engineering. Basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC. Identification of recombinant DNA. Gene expression in microbial (bacteria, yeast) and animal expression systems, markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection).

Specification of controlled education, way of implementation and compensation for absences

Not applicable.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Rosypal S.: Úvod do molekulární biologie I-IV. Brno, 2002. (CS)
e-learning FCH (CS)

Recommended reading

Alberts a kol.: Základy buněčné biologie. Espero Publishing, 2001. (CS)

Classification of course in study plans

Type of course unit

 

Lecture

13 hod., optionally

Teacher / Lecturer

prof. RNDr. Ivana Márová, CSc.

Seminar

26 hod., compulsory

Teacher / Lecturer

prof. RNDr. Ivana Márová, CSc.