Czech

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Theoretical aspects of various experimental techniques of DNA analysis

The lectures are followed by Laboratory course of molecular biotechnology

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The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Examination is oral

1. Methods of cell analysis. Isolation of nucleic acids. Spectrophotometric evaluation of DNA quality.
2. Separation of nucleic acids by electromigration and chromatographic methods.
3. DNA fragmentation by restriction enzymes. Artificial restriction systems.
4. Polymerase Chain Reaction (PCR).
5. Other amplification methods.
6. DNA/DNA hybridization and DNA probes.
7. Fluorescence in situ hybridization - FISH. Chips.
8. Chemical synthesis of DNA.
9. Sequencing of nucleic acids.
10. Carriers with immobilized ligands in molecular diagnostics.
11. Distinguishing of live and dead cells. Application of chromatographic methods in microorganisms identification.
12. QSAR.
13. Bioinformatics (sequences analysis).

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Lectures cover methodologies commonly used in DNA research

Attendance at lectures is not obligatory but it is recommended

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