Czech

Not applicable.

Theoretical aspects of various experimental techniques used in molecular biotechnology research

Pass out the lectures of Molecular genetics I and II is recomended

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Examination is oral

1. Relationship between chemical production and biotechnologies.
2. Transfer of genes using recombinant DNA technology.
3. Cloning vectors and their use.
4. Foreign DNA transfer into cells. Selection of cells with cloned gene.
5. Gene libraries. Cloning of sequences coding eucaryotic proteins.
6. Manipulation of gene expression in prokaryotes.
7. Gene expression from strong and inducible promotors.
8. Fusion proteins. Translation expression vectors. Increasing of protein stability. Integration and shuttle vectors.
9. Production of heterologous proteins in eukaryotic cells.
10. Yeast, insect and mammal expression systems.
11. Biotechnology utilisation of organisms for xenobiotics biodegradation. Microbial insecticides and bacteria stimulating plant growth.
12. Production of therapeutic proteins a vaccines. Gene therapy. Transgenic plants and novel foods. Transgenic animals.
13. Extremophile microorganisms and their biotechnology exploatation.

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Lectures cover methodologies commonly used in molecular biotechnology research

Attendance at lectures is not obligatory but it is recommended

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