Course detail

Molecular Biotechnology

FCH-MC_MOBAcad. year: 2014/2015

Relationship between chemical production and biotechnologies. Transfer of genes using recombinant DNA technology. Cloning vectors and their use. Foreign DNA transfer into cells. Selection of cells with cloned gene. Gene libraries. Cloning of sequences coding eucaryotic proteins. Manipulation of gene expression in prokaryotes. Gene expression from strong and inducible promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Integration and shuttle vectors. Production of heterologous proteins in eukaryotic cells. Yeast, insect and mammal expression systems. Biotechnology utilisation of organisms for xenobiotics biodegradation. Microbial insecticides and bacteria stimulating plant growth. Production of therapeutic proteins a vaccines. Gene therapy. Transgenic plants and novel foods. Transgenic animals. Extremophile microorganisms and their biotechnology exploatation.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Learning outcomes of the course unit

Theoretical aspects of various experimental techniques used in molecular biotechnology research

Prerequisites

Pass out the lectures of Molecular genetics I and II is recomended

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination is oral

Course curriculum

1. Relationship between chemical production and biotechnologies.
2. Transfer of genes using recombinant DNA technology.
3. Cloning vectors and their use.
4. Foreign DNA transfer into cells. Selection of cells with cloned gene.
5. Gene libraries. Cloning of sequences coding eucaryotic proteins.
6. Manipulation of gene expression in prokaryotes.
7. Gene expression from strong and inducible promotors.
8. Fusion proteins. Translation expression vectors. Increasing of protein stability. Integration and shuttle vectors.
9. Production of heterologous proteins in eukaryotic cells.
10. Yeast, insect and mammal expression systems.
11. Biotechnology utilisation of organisms for xenobiotics biodegradation. Microbial insecticides and bacteria stimulating plant growth.
12. Production of therapeutic proteins a vaccines. Gene therapy. Transgenic plants and novel foods. Transgenic animals.
13. Extremophile microorganisms and their biotechnology exploatation.

Work placements

Not applicable.

Aims

Lectures cover methodologies commonly used in molecular biotechnology research

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Not applicable.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NPCP_MA Master's

    branch NPCO_MA , 2 year of study, winter semester, compulsory-optional

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 2 year of study, winter semester, compulsory-optional

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 2 year of study, winter semester, compulsory-optional

  • Programme NPCP_SCH Master's

    branch NPCO_SCH , 2 year of study, winter semester, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer