Course detail

Analytical Chemistry II

FCH-BCA_ANC2Acad. year: 2018/2019

This course aims to introductuion students to the basic methods and techniques of instrumental analytical chemistry. Teaching is structured in the following thematic areas: Physical aspects of analytical methods. Analytical signal and its qulitative and quantitative evaluation. Principles of selected instrumental methods-spectrometric and electroanalytical methods, their qualitative and quantitative aspects and practical examples. Potentiometry. Voltametry and polarography (classical, pulse, stripping). Titrations with polarizable electrodes. Electrogavimetry, coulometry. Conductometry, dielectrimetry. Optical methods, their classification, properties of electromagnetic radiation. Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. Electronic absorption spectra, instrumentation. UV-VIS molecular spectrophotometry. Luinescence methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence. Absorption and emission atomic spectrometry. Principles and importance of chromatographic and electromigration methods. Introduction to analytical chemometry. Evaluation of analytical results. Basics of chemometry. Analytical errors and methods of quantitative evaluation.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

prof. Ing. Josef Čáslavský, CSc.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Learning outcomes of the course unit

By completion of the course the student will gain the following knowledge and skills:
1. They will know in depth the principles of simple instrumental analytical methods and possibilities of their application.
2. They will be able to select the optimal method for solving of specific analytical problems of analytical practice.
3. They will adopt the basic principles of chemometrictreatment of analytical results obtained using simple instrumental-analytical methods.

Prerequisites

Knowledge from the course Analytical chemistry I, knowledge from physics (electricity, magnetism), knowledge from physical chemistry

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Course credit is awarded for 100% attendance at seminars (or properly excused absence). Credit may be obtained for successful completing of three partial written tests during the semester (ie getting at least 6 points out of 10 possible). In case of failure in one partial test (or more) course credit could be awarded for the successful completing of the final test (ie, obtaining at least 60 points out of 100).
Condition for passing the exam is to obtain credit from the exercise of Analytical Chemistry II.
The exam is oral, evaluate the level of knowledge of theory and practice of analytical chemistry and creative approach to solving analytical problems.

Course curriculum

Block 1: Electroanalytical methods
Potentiometry
Voltammetry, polarography (classic, differential pulse, dissolition, adsorptive)
Potentiometric dissolution analysis, titrations with polarizable electrodes¨
Electrogravimetry, coulometry, conductometry.

Block 2: Optical methods
Classification of spectral methods, basic physical principles, properties of electromagnetic radiation
Non-spectral methods: Refractometry, interferometry, polarimetry, nephelometry, turbidimetry
Spectral methods: UV-VIS spectrophotometry - principles, properties, construction of spectrophotometers, applications
Luminescence methods: molecular fluorescence, phosphorescence, chemiluminiscence, bioluminiscence and their analytical applications
Basics of otical atomic spectrometry

Block 3: Principles and applications of basic chromatographic and electromigration techniques
Basic principles of chromatography, separation mechanisms
Chromatographic techniques - paper chromtaography, thin-layer chromatography, column chromatography
Basics of modern liquid and gas chromatography
Separation in electric field - principles, application (electrophoresis)

Work placements

Not applicable.

Aims

The aim of the course is to provide students with an overview of selected instrumental analytical methods from the field of electrochemistry, spectrophotometry and separation techniques and to familiarize them with the strengths and limitations of these techniques. Students will also be introduced to chemometric approaches appropriate for processing the results obtained by these methods.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is recommended, but not controlled. Attendance at seminars is compulsory and is checked.
The students' work is controlled by three partial written tests during the semester in computational exercises.
For students in combined form of study are organized consultations on the topics for full-time students.
Consultations are also provided to full-time students on request.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Kotouček M., Skopalová J.: Příklady z analytické chemie. Vydavatelství Univerzity Palackého Olomouc, 1997. (CS)
Sommer L.: Základy analytické chemie II. VUTIUM, Brno 1998. (CS)
Volka K.: Analytická chemie II. VŠCHT Praha, 1997. (CS)

Type of course unit

Lecture

26 hod., optionally

Teacher / Lecturer

prof. Ing. Josef Čáslavský, CSc.

Exercise

26 hod., compulsory

Teacher / Lecturer

prof. Ing. Josef Čáslavský, CSc.
Mgr. Helena Doležalová Weissmannová, Ph.D.
Ing. Veronika Řezáčová, Ph.D.

VUT

Faculties

University Institutes

Parts

Analytical Chemistry II

Type of course unit