Czech

Not applicable.

Fundamental principles and theoretical knowledge of gene technologies and their biotechnological applications. New strategies in genetic engineering.

Examination - Biochemistry I. Basic knowledge about structure and function of biomolecules.

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week, Seminar - 1 teaching hour per week. The e-learning system (LMS Moodle) is available to teachers and students.

Examination - written and oral part. Elaboration and prezentation of seminar project.

1. Basic terms of molecular genetics; structure and function of information macromolecules
2. Overview of methods for isolation and analysis of nucleic acids
3. Transformation vectors, restriction endonucleases and other enzymes used in recombinant DNA technology
4. Hybridizationof nucleic acids.
5. Methods of transformation, criteria for recombinant cell selection.
6. Clonning of DNA, polymerase chain reaction and its applications in clinical and food diagnostics.
7. Molecular analysis of genome - sequenation. Gene libraries.
8. Mutagenesis.
9. Production, isolation and analysis of recombinant proteins.
10. Construction of transgenic organisms, optimalization of transgene expression.
11. Genetically modified microoorganisms and their applications.
12. Transformation of higher organisms - plants, animals.
13. Overview of applications of genetic engineering in food processing and biotechnology, gene therapy.

Not applicable.

This course is focused on introduction of basic methods and techniques used in gene technology and recombinant DNA technology: isolation, characterization and analysis of nucleic acids, DNA clonning, polymerase chain reaction (PCR), gene mapping, methods of transformation, types of transformation vectors, identification of recombinant DNA, foreign gene expression in prokaryotic and eukaryotic expression systems, identification and purification of expressed recombinant proteins.

Attendance at lectures is not compulsory, but is recommended. Attendance at seminars is compulsory and controlled by lecturer.

Not applicable.

Not applicable.

/e-learning FCH (CS)
Rosypal S.: Úvod do molekulární biologie. Brno, 2002 (CS)

Alberts a kol. : Základy buněčné biologie. Espero Publishong 2001. (CS)