Course detail

Mass Spectrometry

FCH-MC_HMSAcad. year: 2018/2019

Physical bases of mass spectrometry, MS history. Ionization techniques - overview, development, description of contemporary used techniquies (electron ionization, chemical ionization, electrospray, chemical ionization at atmospheric pressure etc). Analyzers in mass spectrometry (static - magnetic, electrostatic, quadrupole, 3D- and linear ion trap, Time-of-Flight, ion cyclotron resonance, orbitrap). Detectors and vacuum systems. Hyphenated and tandem techniquies. Interpretation of mass spectra with respect to the ionization technique applied.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Guarantor

prof. Ing. Josef Čáslavský, CSc.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Learning outcomes of the course unit

Successful completion of the course the student will gain the following knowledge and skills:
1. Students will be familiar with the principles of functions of various types of mass spectrometers;
2. Students will be able to choose the optimal device for highly efficient solutions for specific analytical problems;
3. Students will know the basics of mass spectra interpretation, depending on the ionization technique used and the analyzer resolution.

Prerequisites

Basic knowledge of physics - kinematics, the movement of charged particles in magnetic and electric field
Good knowledge of basic, inorganic, organic, physical and analytical chemistry

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

The exam consists of a written and oral examination. The subject of the written interpretation of the given mass spectrum, the oral examination is focused on the verification of student's knowledge in the range of themes discussed in the lectures. In the final assessment, the result of the written part contributes by 40%, the result of oral part by 60%.

Course curriculum

1. Introduction to mass spectrometry, theoretical basics, natural isotopic distribution of elements and its importance in MS
2. Instrumentation of mass spectrometry
3. Ionization techniques in mass spectrometry
4. Mass analyzers
5. Hyphenated techniques (GC/MS, LC/MS, CE/MS, MS/MS)
6. Interpretation of mass spectra using electron ionization, fragmentation rules
7. Interpretation of mass spectra using soft ionization techniques
8. Practical applications of mass spectrometry

Work placements

Not applicable.

Aims

The aim of this course is:
- to familiarize students with the theoretical foundations of mass spectrometry
- to provide an overview of current instrumentation of mass spectrometry
- to introduce students to the basic concepts interpretation of mass spectra

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is optional and is not controlled, but it is recommended.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

J. H. Gross: Mass Spectrometry, Third Edition, 2017 (EN)
K. Záruba a spol.: Analytická chemie 2. díl, 2016 (CS)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NKCP_CHTOZP Master's

    branch NKCO_CHTOZP , 2 year of study, winter semester, compulsory-optional

  • Programme NPCP_CHTOZP Master's

    branch NPCO_CHTOZP , 2 year of study, winter semester, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

prof. Ing. Josef Čáslavský, CSc.